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Anti-p53 [PAb242]

Invented by David Lane at Karolinska Institutet

Info

Catalogue Number 153297
Applications ELISA IHC IF IP WB
Antigen/Gene or Protein Targets p53
Synonyms Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53
Reactivity Mouse
Relevance p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. PAB 1802 may be used to show the expression of human p53 cDNA in cos-l cells.
Host Mouse
Immunogen SV40-transformed cell line BALB/c SVA31 E7
Positive Control MEFs
Subclass IgG1
Molecular Weight (kDa) 53
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions Cells were grown at 37°C and at 10% (vol/vol) CO2 in air. The cell culture medium was Dulbecco modified Eagle minimum essential medium supplemented with 10% (vol/vol) fetal calf serumplus 500 U of penicillin and 100ug Streptomycin per ml.
Strain Balb/c
Notes This monoclonal works well on paraffin sections of mouse tissues.
The epitope of has been mapped to the sequence 18-TFSGLWKLLP-27 in the N-terminal domain of mouse p53 (Yewdell et al., 1986; Lane et al., 1996).
Research Area Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, DNA Damage and Repair, Epigenetics & Nuclear Signalling

References

There are 6 reference entries for this reagent.

View All References

References: 6 entries

Eng et al. 2016. Protein Eng Des Sel. 29(1):11-21. PMID: 26508747.

IHC WB

Enhanced antigen detection in immunohistochemical staining using a 'digitized' chimeric antibody.

Europe PMC ID: 26508747

Lane et al. 1996. Oncogene. 12(11):2461-6. PMID: 8649788.

ELISA

Epitope analysis of the murine p53 tumour suppressor protein.

Europe PMC ID: 8649788

Yewdell et al. 1986. J Virol. 59(2):444-52. PMID: 2426467.

IP WB

Monoclonal antibody analysis of p53 expression in normal and transformed cells.

Europe PMC ID: 2426467


Add a reference

References: 6 entries

Eng et al. 2016. Protein Eng Des Sel. 29(1):11-21. PMID: 26508747.

IHC WB

Enhanced antigen detection in immunohistochemical staining using a 'digitized' chimeric antibody.

Lane et al. 1996. Oncogene. 12(11):2461-6. PMID: 8649788.

ELISA

Epitope analysis of the murine p53 tumour suppressor protein.

Yewdell et al. 1986. J Virol. 59(2):444-52. PMID: 2426467.

IP WB

Monoclonal antibody analysis of p53 expression in normal and transformed cells.


Add a reference