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Anti-p53 [PAb242]

Invented by David Lane at Imperial College

Info

Catalogue Number 153297
Applications ELISA IHC IF IP WB
Antigen/Gene or Protein Targets p53
Synonyms Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53
Reactivity Mouse
Relevance Monoclonal antibody directed against p53, binding a distinct epitope in amino acids 18-27 at the N-terminus.

Background and Research Application
Anti-p53 binds p53 protein, a crucial tumour suppressor protein, implicated in over 50% of cancers. p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. p53 is involved in many mechanisms of anti-cancer function, and plays a role in apoptosis, genomic stability, cell cycle regulation and inhibition of angiogenesis. p53 is known as the guardian of the genome as it functions to prevent gene mutation. Mutants of p53 which frequently occur in human cancers, fail to recognise their DNA binding site, leading to the loss of tumour suppressor activity. p53 is typically found in low levels in healthy cells due to its short half-life, however in disease states it is expressed in high amounts due to somatic mutations and believed to contribute to transformation and malignancy. p53 mutations are found in over 50% of cancers with germline mutations, offering a predisposition to inherited cancer susceptibility syndrome.

Understanding the functions of mutant p53 can help in the development of new therapeutic approaches that may be useful in a broad range of cancer types. Anti-p53 Pab242 binds to an epitope within amino acids 18-27 at the N-terminus.
Host Mouse
Immunogen SV40-transformed cell line BALB/c SVA31 E7
Immunogen UniProt ID P04637
Positive Control MEFs
Subclass IgG1
Molecular Weight (kDa) 53
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions Cells were grown at 37°C and at 10% (vol/vol) CO2 in air. The cell culture medium was Dulbecco modified Eagle minimum essential medium supplemented with 10% (vol/vol) fetal calf serumplus 500 U of penicillin and 100ug Streptomycin per ml.
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Anti-p53 Pab242 binding to p53 is not inhibited by any other anti-p53 antibodies, as it binds to a sterically discrete epitope.

This monoclonal works well on paraffin sections of mouse tissues.
The epitope of has been mapped to the sequence 18-TFSGLWKLLP-27 in the N-terminal domain of mouse p53 (Yewdell et al., 1986; Lane et al., 1996).

Concentration
1mg/ml as standard
Research Area Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, DNA Damage and Repair, Epigenetics & Nuclear Signalling

References

There are 8 reference entries for this reagent.

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References: 8 entries

Sabapathy et al. 2019. J Mol Cell Biol. 11(4):317-329. PMID: 30907951.

Eng et al. 2016. Protein Eng Des Sel. 29(1):11-21. PMID: 26508747.

IHC WB

Enhanced antigen detection in immunohistochemical staining using a 'digitized' chimeric antibody.

Europe PMC ID: 26508747

Mayelzadeh et al. 2007. Oncogene. 26(21):2955-63. PMID: 17130840.

Lane et al. 1996. Oncogene. 12(11):2461-6. PMID: 8649788.

ELISA

Epitope analysis of the murine p53 tumour suppressor protein.

Europe PMC ID: 8649788

Yewdell et al. 1986. J Virol. 59(2):444-52. PMID: 2426467.

IP WB

Monoclonal antibody analysis of p53 expression in normal and transformed cells.

Europe PMC ID: 2426467


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References: 8 entries

Sabapathy et al. 2019. J Mol Cell Biol. 11(4):317-329. PMID: 30907951.

Eng et al. 2016. Protein Eng Des Sel. 29(1):11-21. PMID: 26508747.

IHC WB

Enhanced antigen detection in immunohistochemical staining using a 'digitized' chimeric antibody.

Mayelzadeh et al. 2007. Oncogene. 26(21):2955-63. PMID: 17130840.

Lane et al. 1996. Oncogene. 12(11):2461-6. PMID: 8649788.

ELISA

Epitope analysis of the murine p53 tumour suppressor protein.

Yewdell et al. 1986. J Virol. 59(2):444-52. PMID: 2426467.

IP WB

Monoclonal antibody analysis of p53 expression in normal and transformed cells.


Add a reference