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DOK Cell Line

Info

Catalogue Number 153251
Host Human
Tissue Tongue
Disease Keywords Human Caucasian dysplastic oral keratinocyte
Model Tumour line
Relevance The degree of dysplasia in the patient was described as mild to moderate. The cells show some stratification in confluent cultures and contain a keratin profile similar to the original dysplasia. Growth is stimulated by hydrocortisone and inhibited by cholera toxin. Expression of p53 is increased and a 12 bp in-frame deletion of the p53 gene (codon 188-191) could be identified. DOK cells are non-tumourigenic in athymic nude mice.
Production Details The dysplastic oral keratinocyte cell line DOK was isolated from a piece of dorsal tongue from a 57 year old man. After a squamous-cell carcinoma was removed from the patient, who had been a heavy smoker, the remaining dysplasia was removed subsequently and used to initiate primary cultures leading to the establishment of DOK.
Conditional No
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Adherent
Recommended Growing Conditions Subculture Routine: Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Culture Medium: DMEM + 2mM Glutamine + 5µg/ml Hydrocortisone + 10% Foetal Bovine Serum (FBS).
Notes STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,13
D16S539: 9,12
D5S818: 12
D7S820: 11,13
THO1: 6,8
TPOX: 10
vWA: 14,18
Cellosaurus ID CVCL_1180

References

There are 4 reference entries for this reagent.

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References: 4 entries

Burns et al. 1994. Br J Cancer. 70(4):591-5. PMID: 7917902.

The p53 status of cultured human premalignant oral keratinocytes.

Europe PMC ID: 7917902

Chang et al. 1992. Int J Cancer. 52(6):896-902. PMID: 1459732.

DOK, a cell line established from human dysplastic oral mucosa, shows a partially transformed non-malignant phenotype.

Europe PMC ID: 1459732


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References: 4 entries

Burns et al. 1994. Br J Cancer. 70(4):591-5. PMID: 7917902.

The p53 status of cultured human premalignant oral keratinocytes.

Chang et al. 1992. Int J Cancer. 52(6):896-902. PMID: 1459732.

DOK, a cell line established from human dysplastic oral mucosa, shows a partially transformed non-malignant phenotype.


Add a reference

Inventor Information