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Pr8/22 Cell Line
RECENTLY UPDATED

Invented at Cancer Research UK Manchester Institute

Info

Catalogue Number 153248
Parental Line P388
Host Mouse
Tissue Lymphatic Tissue
Disease Keywords Drug resistant
Model Tumour line
Relevance The cell line Pr8/22 has been derived from P388, a methylcholanthrene-induced lymphoid neoplasm originating in DBA/2 mouse and converted to ascitic form in the first mouse transfer. Pr8/22 is resistant to daunorubicin and is multi drug resistant (MDR). Cells should be treated with the drug at least once a month and before freezing, however after resuscitating daunorubicin should not be added until the first passage. It is advisable to keep a backup culture which is not drug-challenged.
Production Details The cell line Pr8/22 has been derived from P388, a methylcholanthrene-induced lymphoid neoplasm.
Conditional No
Research Area Cancer, DNA Damage and Repair, Drug Discovery & Development
Growth/Phenotype Keywords Suspension
Recommended Growing Conditions Sub-culture routine: Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C. It is recommended to culture the cells without drug upon resuscitation until the first passage.Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Horse Serum (HS); treat once a month with 0.1µM Daunorubicin.
Cellosaurus ID CVCL_4276

References

There are 2 reference entries for this reagent.

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References: 2 entries

McGown et al. 1983. Cancer Chemother Pharmacol. 11(2):113-6. PMID: 6627597.

Comparative studies of the uptake of daunorubicin in sensitive and resistant P388 cell lines by flow cytometry and biochemical extraction procedures.

Europe PMC ID: 6627597


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References: 2 entries

McGown et al. 1983. Cancer Chemother Pharmacol. 11(2):113-6. PMID: 6627597.

Comparative studies of the uptake of daunorubicin in sensitive and resistant P388 cell lines by flow cytometry and biochemical extraction procedures.


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