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fR5 Cell Line

Invented by Prof Joyce Taylor-Papadimitriou at King's College London, Dr Sidney Chang at Retired Consultant

Info

Catalogue Number 153247
Host Human
Tissue Breast
Disease Keywords Breast cancer
Model Immortalised Line
Relevance fR5 cells do not express Muc-1, also known as HMFG-1 antigen, which is a mucin-like component of human milk fat globule membranes. They are negative for keratins 4, 6, 7, 8, 10, 13 14, 16, 17, 18 and 19. Although fR5 is morphologically different from fR2, they have probably been derived from a common precursor cell since the karyotypes are similar. Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions. fR5 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumourigenic in nude mice.
Production Details The breast epithelial cell line fR5 was established in 1982 by infecting suspensions of primary milk cultures with wild-type SV40.
Conditional No
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Adherent
Recommended Growing Conditions Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 1 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture Medium: RPMI 1640 + 2mM Glutamine + 10µg/ml insulin + 5µg/ml hydrocortisone + 10% FBS
Cellosaurus ID CVCL_2445

References

There are 2 reference entries for this reagent.

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References: 2 entries

Chang et al. 1982. Cancer Res. 42(5):2040-53. PMID: 6279290.

Establishment and characterization of SV40-transformed human breast epithelial cell lines.

Europe PMC ID: 6279290


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References: 2 entries

Chang et al. 1982. Cancer Res. 42(5):2040-53. PMID: 6279290.

Establishment and characterization of SV40-transformed human breast epithelial cell lines.


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