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fR2 Cell Line

Invented by Prof Joyce Taylor-Papadimitriou at King's College London, Dr Sidney Chang at Retired Consultant

Info

Catalogue Number 153246
Host Human
Tissue Breast
Disease Keywords Breast cancer
Model Immortalised Line
Relevance fR2 cells have been shown to express Muc-1, also known as HMFG-1 antigen, which is a mucin-like component of human milk fat globule membranes. They are positive for keratin 8 and 18, but negative for keratins 4, 6, 7, 10, 13, 14, 16, 17 and 19. Although fR2 is morphologically different from fR5, they have probably been derived from a common precursor cell since the karyotypes are similar. Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions. fR2 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumorigenic in nude mice.
Production Details The breast epithelial cell line fR2 was established in 1982 by infecting suspensions of primary milk cultures with wild-type SV40.
Conditional No
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Adherent
Recommended Growing Conditions Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 1 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture Medium: RPMI 1640 + 2mM Glutamine + 10µg/ml insulin + 5µg/ml hydrocortisone + 10% FBS
Notes STR-PCR Data:
Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 11,13
D5S818: 12,13
D7S820: 8,12
THO1: 9.3
TPOX: 8
vWA: 20
Cellosaurus ID CVCL_2444

References

There are 2 reference entries for this reagent.

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References: 2 entries

Chang et al. 1982. Cancer Res. 42(5):2040-53. PMID: 6279290.

Establishment and characterization of SV40-transformed human breast epithelial cell lines.

Europe PMC ID: 6279290


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References: 2 entries

Chang et al. 1982. Cancer Res. 42(5):2040-53. PMID: 6279290.

Establishment and characterization of SV40-transformed human breast epithelial cell lines.


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