RPE1 FRT/TR Cell Line
Invented by Dr Jonathon Pines at University of Cambridge
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 153242 |
| Parental Line | RPE1 |
| Host | Human |
| Tissue | Eye |
| Relevance | This cell line can be used to generate cell lines with tetracycline inducible expression of genes of interest. The cell line has a randomly integrated FRT locus and Tet repressor for Flp-In expression. For example the originator of the cell line used this cell line to generate tetracycline-inducible cell lines expressing APC15-IRES2-mRuby, APC15-3xflag and Cyclin B1-L45A-HA using the FLIP-in system and a modified pCDNA5/FRT/TO vector. |
| Production Details | This tetracycline-inducible RPE1 cell line was created by random integration of an FRT site and a Tet repressor gene into retinal pigment epithelial 1 (RPE1) cells. |
| Conditional | Yes |
| Conditional Description | Tetracycline-inducible expression of genes of interest. |
| Research Area | Gene Expression, Genetic Studies Tools |
| Growth/Phenotype Keywords | Adherent cell line |
| Recommended Growing Conditions | F12:DMEM (1:1) media supplemented with 10% FCS |
References: 2 entries
Mansfeld et al. 2011. Nat Cell Biol. 13(10):1234-43. PMID: 21926987.
APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.
Europe PMC ID: 21926987
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References: 2 entries
Mansfeld et al. 2011. Nat Cell Biol. 13(10):1234-43. PMID: 21926987.
APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.
Add a reference
