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RPE1 FRT/TR Cell Line

Invented by Dr Jonathon Pines at Gurdon Institute

Info

Catalogue Number 153242
Parental Line RPE1
Host Human
Tissue Eye
Relevance This cell line can be used to generate cell lines with tetracycline inducible expression of genes of interest. The cell line has a randomly integrated FRT locus and Tet repressor for Flp-In expression. For example the originator of the cell line used this cell line to generate tetracycline-inducible cell lines expressing APC15-IRES2-mRuby, APC15-3xflag and Cyclin B1-L45A-HA using the FLIP-in system and a modified pCDNA5/FRT/TO vector.
Production Details This tetracycline-inducible RPE1 cell line was created by random integration of an FRT site and a Tet repressor gene into retinal pigment epithelial 1 (RPE1) cells.
Conditional Yes
Conditional Description Tetracycline-inducible expression of genes of interest.
Research Area Gene Expression, Genetic Studies Tools
Growth/Phenotype Keywords Adherent cell line
Recommended Growing Conditions F12:DMEM (1:1) media supplemented with 10% FCS

References

There are 2 reference entries for this reagent.

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References: 2 entries

Mansfeld et al. 2011. Nat Cell Biol. 13(10):1234-43. PMID: 21926987.

APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.

Europe PMC ID: 21926987


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References: 2 entries

Mansfeld et al. 2011. Nat Cell Biol. 13(10):1234-43. PMID: 21926987.

APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.


Add a reference