RPE1 FRT/TR Cell Line
Invented by Dr Jonathon Pines from Gurdon Institute
Invented at University of Cambridge
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 153242 |
| Parental Line | RPE1 |
| Host | Human |
| Tissue | Eye |
| Relevance | This cell line can be used to generate cell lines with tetracycline inducible expression of genes of interest. The cell line has a randomly integrated FRT locus and Tet repressor for Flp-In expression. For example the originator of the cell line used this cell line to generate tetracycline-inducible cell lines expressing APC15-IRES2-mRuby, APC15-3xflag and Cyclin B1-L45A-HA using the FLIP-in system and a modified pCDNA5/FRT/TO vector. |
| Production Details | This tetracycline-inducible RPE1 cell line was created by random integration of an FRT site and a Tet repressor gene into retinal pigment epithelial 1 (RPE1) cells. |
| Conditional | Yes |
| Conditional Description | Tetracycline-inducible expression of genes of interest. |
| Research Area | Gene Expression, Genetic Studies Tools |
| Growth/Phenotype Keywords | Adherent cell line |
| Recommended Growing Conditions | F12:DMEM (1:1) media supplemented with 10% FCS |
| Notes | This cell line is resistant to Hygromycin (hTERT), Zeocin (FRT), Blasticidin (TR) and Puromycin (hTERT). The hygromycin resistance cassette in the original plasmid was replaced with neomycin resistance. For selection after the Flp-In integration the inventors used Geneticin G418 sulphate (an analog of neomycin sulphate) at a final concentration of 0.5 mg/mL. |
References: 2 entries
Mansfeld et al. 2011. Nat Cell Biol. 13(10):1234-43. PMID: 21926987.
APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.
Europe PMC ID: 21926987
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References: 2 entries
Mansfeld et al. 2011. Nat Cell Biol. 13(10):1234-43. PMID: 21926987.
APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.
Add a reference
