![Immunoblot to detect Cdc25. Anti-Cdc25 [DG4] and [DG9] detect only the fast migrating un-phosphorylated Cdc25. Anti-Cdc25 [DG6] and [DG20] detect the fast migrating un-phosphorylated Cdc25 and the slower phosphorylated Cdc25.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/f79471da-fc3e-45bb-bb13-20438375244e.jpg)
![Immunoblot to detect Cdc25. Anti-Cdc25 [DG4] and [DG9] detect only the fast migrating un-phosphorylated Cdc25. Anti-Cdc25 [DG6] and [DG20] detect the fast migrating un-phosphorylated Cdc25 and the slower phosphorylated Cdc25.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/f79471da-fc3e-45bb-bb13-20438375244e.jpg)
![Image thumbnail for Anti-Non-phospho Cdc25 [DG6]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/f79471da-fc3e-45bb-bb13-20438375244e.jpg)
Anti-Cdc25 [DG4]
Invented by Julian Gannon at Cancer Research UK London Research Institute: Clare Hall Laboratories
![Immunoblot to detect Cdc25. Anti-Cdc25 [DG4] and [DG9] detect only the fast migrating un-phosphorylated Cdc25. Anti-Cdc25 [DG6] and [DG20] detect the fast migrating un-phosphorylated Cdc25 and the slower phosphorylated Cdc25.](https://res.cloudinary.com/ximbio/image/upload/c_thumb,fl_lossy,h_124,q_auto,r_max,w_124/c059fd8e-d815-45ff-b8a8-6102862c36ba.jpg)
Anti-Non-phospho Cdc25 [DG6]
Invented by Julian Gannon from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (0)
- Inventor Info
Info
| Catalogue Number | 153196 |
| Applications | WB |
| Antigen/Gene or Protein Targets | Cdc25 |
| Synonyms | Cell Division Cycle 25, CTN1 |
| Reactivity | Xenopus laevis |
| Relevance | Cdc25 (cell division cycle 25) is a dual-specificity phosphatase that controls entry into and progression through various phases of the cell cycle, including mitosis and S phase. |
| Host | Mouse |
| Immunogen | Recombinant Xenopus Cdc25 |
| Positive Control | Xenopus egg extract |
| Notes |
Cdc25 is very abundant in egg extracts. In mitiotic extracts Cdc25 is phosphorylated which slowers it’s migration in SDS gels. The epitope recognised by DG4 and DG9 is not present on the mitotic form of Cdc25. Presumably these antibodies recognise an unphosphorylated epitope. Phosphorylation masks the epitope and the antibody is unable to recognise Cdc25. The epitope recognised by DG6 and DG20 is present in all forms of Cdc25. |
| Research Area | Cancer, Cell Cycle, Epigenetics & Nuclear Signalling |
References
There are 0 reference entries for this reagent.
![Immunoblot to detect Cdc25. Anti-Cdc25 [DG4] and [DG9] detect only the fast migrating un-phosphorylated Cdc25. Anti-Cdc25 [DG6] and [DG20] detect the fast migrating un-phosphorylated Cdc25 and the slower phosphorylated Cdc25.](https://res.cloudinary.com/ximbio/image/upload/c_thumb,fl_lossy,h_124,q_auto,r_max,w_124/fcdb76d3-9fa6-451f-93f1-06ec62a36f18.jpg)
![Immunoblot to detect Cdc25. Anti-Cdc25 [DG4] and [DG9] detect only the fast migrating un-phosphorylated Cdc25. Anti-Cdc25 [DG6] and [DG20] detect the fast migrating un-phosphorylated Cdc25 and the slower phosphorylated Cdc25.](https://res.cloudinary.com/ximbio/image/upload/c_thumb,fl_lossy,h_124,q_auto,r_max,w_124/44af5973-2d06-41f7-853a-d5902b0817e5.jpg)
References: 0 entry
There is no reference for this reagent yet, feel free to use the button below to suggest one.
Add a reference
References: 0 entry
There is no reference for this reagent yet, feel free to use the button below to suggest one.
Add a reference
