Invented by Julian Gannon at Cancer Research UK London Research Institute: Clare Hall Laboratories
|Antigen/Gene or Protein Targets||Cdc25|
|Synonyms||Cell Division Cycle 25, CTN1|
|Relevance||Cdc25 (cell division cycle 25) is a dual-specificity phosphatase that controls entry into and progression through various phases of the cell cycle, including mitosis and S phase.|
|Immunogen||Recombinant Xenopus Cdc25|
|Positive Control||Xenopus egg extract|
Cdc25 is very abundant in egg extracts. In mitiotic extracts Cdc25 is phosphorylated which slowers it’s migration in SDS gels.
The epitope recognised by DG4 and DG9 is not present on the mitotic form of Cdc25. Presumably these antibodies recognise an unphosphorylated epitope. Phosphorylation masks the epitope and the antibody is unable to recognise Cdc25.
The epitope recognised by DG6 and DG20 is present in all forms of Cdc25.
|Research Area||Cancer, Cell Cycle, Epigenetics & Nuclear Signalling|
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