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Anti-Non-phospho Cdc25 [DG6]
RECENTLY UPDATED

Invented by Julian Gannon from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories

Info

Catalogue Number 153196
Applications WB
Antigen/Gene or Protein Targets Cdc25
Synonyms Cell Division Cycle 25, CTN1
Reactivity Xenopus laevis
Relevance Cdc25 (cell division cycle 25) is a dual-specificity phosphatase that controls entry into and progression through various phases of the cell cycle, including mitosis and S phase.
Host Mouse
Immunogen Recombinant Xenopus Cdc25
Positive Control Xenopus egg extract
Notes Cdc25 is very abundant in egg extracts. In mitiotic extracts Cdc25 is phosphorylated which slowers it’s migration in SDS gels.

The epitope recognised by DG4 and DG9 is not present on the mitotic form of Cdc25. Presumably these antibodies recognise an unphosphorylated epitope. Phosphorylation masks the epitope and the antibody is unable to recognise Cdc25.

The epitope recognised by DG6 and DG20 is present in all forms of Cdc25.
Research Area Cancer, Cell Cycle, Epigenetics & Nuclear Signalling

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