Anti-Acetyl SMC3 [EM5.5.2]
Invented by Julian Gannon from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
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![Figure. Immoblots using Anti-Acetyl SMC3 [EM5.5.2] and Anti-SMC3 [DJ5.2.3]. DJ5.2.3 immunoblots all forms of SMC3 while EM5.5.2 immunoblots only the Acetyl SMC3.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/ad1e90ac-077e-4f07-ba49-3f3553197a23.jpg)
![Figure. Immoblots using Anti-Acetyl SMC3 [EM5.5.2] and Anti-SMC3 [DJ5.2.3]. DJ5.2.3 immunoblots all forms of SMC3 while EM5.5.2 immunoblots only the Acetyl SMC3.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/ad1e90ac-077e-4f07-ba49-3f3553197a23.jpg)
![Image thumbnail for Anti-Acetyl SMC3 [EM5.5.2]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/ad1e90ac-077e-4f07-ba49-3f3553197a23.jpg)
| Catalogue Number | 153181 |
| Applications | WB |
| Antigen/Gene or Protein Targets | Acetyl SMC3 |
| Reactivity | Xenopus laevis |
| Relevance |
SMC3 is a central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement. SMC3 is unacetylated in egg extracts. It is only acetylated in chromatin preparations from extracts where the DNA has replicated. |
| Host | Mouse |
| Immunogen | Synthetic peptide - EEVSLRRVIGAKacetyl Kacetyl DQYFLDKK |
| Positive Control | Xenopus chromatin extract |
| Molecular Weight (kDa) | 141 |
| Notes |
Raised from mice immunized with the peptide sequence: EEVSLRRVIGAKacetyl Kacetyl DQYFLDKK corrosponding to amino acids 94 -114 of Xenopus laevis SMC3 The epitope recognised by these antibodies is identical to the human sequence amino acid 98 -114 Human [LRRVIGAKacetyl Kacetyl DQYFLDKK] (Lys 105, Lys 106) Xenopus [LRRVIGAKacetyl Kacetyl DQYFLDKK] EM5.5.2 reacts against Xenopus Acetyl SMC3. Not tested against human and mouse. |
| Research Area | Cancer, Cell Cycle, DNA Damage and Repair, Epigenetics & Nuclear Signalling |
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