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Anti-Acetyl SMC3 [EN9.1]

Invented by Julian Gannon at The Francis Crick Institute

Info

Catalogue Number 153175
Applications WB
Antigen/Gene or Protein Targets Acetyl SMC3
Reactivity Xenopus laevis
Relevance SMC3 is a central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.

SMC3 is unacetylated in egg extracts. It is only acetylated in chromatin preparations from extracts where the DNA has replicated.
Host Mouse
Immunogen Synthetic peptide - EEVSLRRVIGAKacetyl Kacetyl DQYFLDKK
Positive Control Xenopus chromatin extract
Molecular Weight (kDa) 141
Notes Raised from mice immunized with the peptide sequence:
EEVSLRRVIGAKacetyl Kacetyl DQYFLDKK
corrosponding to amino acids 94 -114 of Xenopus laevis SMC3

The epitope recognised by these antibodies is identical to the human sequence amino acid 98 -114:
Human [LRRVIGAKacetyl Kacetyl DQYFLDKK] (Lys 105, Lys 106)
Xenopus [LRRVIGAKacetyl Kacetyl DQYFLDKK]

EN9.1 reacts against Xenopus Acetyl SMC3. Not tested against human and mouse.
EN9.1 gives the strongest signal compared to DP3.1, DP4.1 and EM5.5.2 clones.
Research Area Cancer, Cell Cycle, DNA Damage and Repair, Epigenetics & Nuclear Signalling

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