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Nes-CreER(T2)(+);Idh1fl(R132H)/+ Mouse

Invented by Prof Ian Tomlinson at University of Oxford

Info

Antigen/Gene or Protein Targets Isocitrate dehydrogenase 1
Disease Keywords Glioma
Synonyms IDH1, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase
Model Knock-In
Relevance Isocitrate dehydrogenase 1 R132H mutations drive the growth of human gliomas, probably through neomorphic enzyme activity that produces 2-hydroxyglutarate (2HG). The Nes-CreER(T2)(+);Idh1fl(R132H)/+ mouse is a tool to model this disease, enabling inducible conditional expression of Idh1R132H in the subventricular zone (SVZ) of the adult mouse brain.
Conditional Yes
Growth/Phenotype Keywords Idh1R132H mutant
Strain C57BL/6
Research Area Cancer, Neurobiology
Notes Expression of the Idh1R132H mutant protein can be induced by giving the mice tamoxifen. The mice carry a tamoxifen-inducible Cre, Nestin-CreER(T2) which in adult mice targets Cre to the subventricular zone (SVZ) and the other major neurogenic niche, the subgranular zone (SGZ) of the hippocampal dentate gyrus. When induced using tamoxifen to express the Idh1R132H mutant protein the mice are referred to as Tam-Idh1-KI mice.

References

There are 2 reference entries for this reagent.

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References: 2 entries

Bardella et al. 2016. Cancer Cell. 30(4):578-594. PMID: 27693047.

Expression of Idh1R132H in the Murine Subventricular Zone Stem Cell Niche Recapitulates Features of Early Gliomagenesis.

Europe PMC ID: 27693047


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References: 2 entries

Bardella et al. 2016. Cancer Cell. 30(4):578-594. PMID: 27693047.

Expression of Idh1R132H in the Murine Subventricular Zone Stem Cell Niche Recapitulates Features of Early Gliomagenesis.


Add a reference