- References (2)
- Inventor Info
|Antigen/Gene or Protein Targets||N-acetylgalactosaminide alpha-2,6-sialyltransferase 1 - ST6GalNAc-1|
|Disease Keywords||Breast Cancer|
The parental T47D cell line was established from the pleural effusion of a ductal carcinoma of the breast. The tumour cell line, T47D-STn, was created as a breast cancer model which expresses the Sialyl-Tn, STn, antigen.
T47D-STn cells overexpress the enzymes C2GnT1, a beta-1,3galactosyl-O-glycosyl-glycoprotein and glycosyltransferase, ST6GalNAc-I.
ST6GalNAc-I which is found throughout the Golgi, can catalyse the transfer of sialic acid to N-acetylgalactosamine and is predominately limited to the gastrointestinal tract in normal adult tissues. Expression of ST6GalNAc-I in human cells promotes formation of the STn antigen which is a saccharide structure (N-acetylgalactosamine) linked to serine or threonine by a glycosidic bond. STn antigens are not usually found on healthy cell surfaces but often on human breast cancer cells.
Together with wild-type T47D and T47D-E2J these cell lines represent a panel of isogenic cells with different O-linked glycosylation patterns.
|Research Area||Cancer, Drug Discovery & Development|
|Recommended Growing Conditions||DMEM 10% FCS, 500µg/ml G418, 300µg/ml hygromycin|
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