Cancer Research Technology
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Catalogue Number 152840
Parental Line NGP
Host Human
Tissue Lung
Disease Keywords Neuroblastoma
Model Cancer Model
Relevance To determine how resistance to MDM2/p53 binding antagonists might develop, NGP cells were exposed to growth inhibitory concentrations of a MDM2 inhibitor, Nutlin-3, and a clonal resistant cell line was generated. The p53 mediated responses of the parental and resistant cell line were compared. In contrast to the parental cell lines, p53 activation by Nutlin-3, MI-63 or ionizing radiation was not observed in the NGP derived cell line, N_N20R1.
Production Details Resistant cell lines were established by exposing NGP cells to Nutlin-3. Single cell derived colonies were isolated with cloning cylinders and the clonal population expanded in culture medium containing the MDM2/p53 antagonist refreshed weekly for 60 days. Stage 1 resistant clones were then further exposed to increased concentrations of Nutlin-3 for 30 days to generate stage 2 resistant clones.
Conditional No
Research Area Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, Drug Discovery & Development, Epigenetics & Nuclear Signalling

References: 2 entries

TP53 mutant MDM2-amplified cell lines selected for resistance to MDM2-p53 binding antagonists retain sensitivity to ionizing radiation.

Europe PMC ID: 27323823

Drummond et al. 2016. Oncotarget. :. PMID: 27323823.


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References: 2 entries

TP53 mutant MDM2-amplified cell lines selected for resistance to MDM2-p53 binding antagonists retain sensitivity to ionizing radiation.

Europe PMC ID: 27323823

Drummond et al. 2016. Oncotarget. :. PMID: 27323823.


Add a reference

References: 2 entries

TP53 mutant MDM2-amplified cell lines selected for resistance to MDM2-p53 binding antagonists retain sensitivity to ionizing radiation.

Drummond et al. 2016. Oncotarget. :. PMID: 27323823.


Add a reference


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