N_M5R1 Cell Line
Invented at University of Newcastle upon Tyne
- References (2)
- Inventor Info
|To determine how resistance to MDM2/p53 binding antagonists might develop, NGP cells were exposed to growth inhibitory concentrations of a MDM2 inhibitor, MI-63, and a clonal resistant cell line was generated. The p53 mediated responses of the parental and resistant cell line were compared. In contrast to the parental cell lines, p53 activation by Nutlin-3, MI-63 or ionizing radiation was not observed in the NGP derived cell line, N_M5R1.
|Resistant cell lines were established by exposing NGP cells to MI-63. Single cell derived colonies were isolated with cloning cylinders and the clonal population expanded in culture medium containing the MDM2/p53 antagonist refreshed weekly for 60 days. Stage 1 resistant clones were then further exposed to increased concentrations of MI-63 for 30 days to generate stage 2 resistant clones.
|Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, Drug Discovery & Development, Epigenetics & Nuclear Signalling
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