Anti-CIDE A [V62P1E3*B10]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
- References (2)
- Inventor Info
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![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human breast cancer sections using anti-CIDE A [V62P1E3*B10]. Positive cytoplasmic staining is shown.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/d8b0544a-f611-4bf6-94af-dd9ef618ac12.jpg)
![Western blotting was performed on lysates prepared from cells overexpressing CIDE A (+) and cells transfected with an empty vector (-) using anti-CIDE A [V62P1E3*B10].](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/f937771c-4975-468c-bd45-b7fc512d0bb5.jpg)
![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections. Strong cytoplasmic positivity is observed in primary colorectal cancer using anti-CIDEA antibody [V62P1E3*B10]. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/210a5251-a3c5-4b31-a68d-258ecb5b2b6a.jpg)
![IHC photomicrographs showing weak cytoplasmic staining of Anti-CIDE A [V62P1E3*B10] in normal colon mucosa. IHC was performed on formalin-fixed, paraffin-embedded normal colon mucosa.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/e6c6d5c9-bfad-44bd-9451-3b8bd3b4afa0.jpg)
![Survival curve comparing patients outcome categorised by expression of CIDEA Anti-CIDE A [V62P1E3*B10]. Positive expression of CIDEA was associated with improved patients’ outcome (p=0.004).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/2d5eb3f2-74ba-4808-ab89-6a456e161fd5.png)
![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human breast cancer sections using anti-CIDE A [V62P1E3*B10]. Positive cytoplasmic staining is shown.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/d8b0544a-f611-4bf6-94af-dd9ef618ac12.jpg)
![Western blotting was performed on lysates prepared from cells overexpressing CIDE A (+) and cells transfected with an empty vector (-) using anti-CIDE A [V62P1E3*B10].](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/f937771c-4975-468c-bd45-b7fc512d0bb5.jpg)
![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections. Strong cytoplasmic positivity is observed in primary colorectal cancer using anti-CIDEA antibody [V62P1E3*B10]. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/210a5251-a3c5-4b31-a68d-258ecb5b2b6a.jpg)
![IHC photomicrographs showing weak cytoplasmic staining of Anti-CIDE A [V62P1E3*B10] in normal colon mucosa. IHC was performed on formalin-fixed, paraffin-embedded normal colon mucosa.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/e6c6d5c9-bfad-44bd-9451-3b8bd3b4afa0.jpg)
![Survival curve comparing patients outcome categorised by expression of CIDEA Anti-CIDE A [V62P1E3*B10]. Positive expression of CIDEA was associated with improved patients’ outcome (p=0.004).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/2d5eb3f2-74ba-4808-ab89-6a456e161fd5.png)
![Image thumbnail for Anti-CIDE A [V62P1E3*B10]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/d8b0544a-f611-4bf6-94af-dd9ef618ac12.jpg)
![Image thumbnail for Anti-CIDE A [V62P1E3*B10]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/f937771c-4975-468c-bd45-b7fc512d0bb5.jpg)
![Image thumbnail for Anti-CIDE A [V62P1E3*B10]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/210a5251-a3c5-4b31-a68d-258ecb5b2b6a.jpg)
![Image thumbnail for Anti-CIDE A [V62P1E3*B10]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/e6c6d5c9-bfad-44bd-9451-3b8bd3b4afa0.jpg)
![Image thumbnail for Anti-CIDE A [V62P1E3*B10]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/2d5eb3f2-74ba-4808-ab89-6a456e161fd5.png)
| Catalogue Number | 152792 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Cell Death-Inducing DFFA-Like Effector A (CIDE A) |
| Synonyms | Cell death activator CIDE A, Cell Death Inducing DFFA Like Effector A, cell death inducing DNA fragmentation factor, alpha subunit like effector A, CIDE-A, CIDEA |
| Reactivity | Human |
| Relevance | Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICARD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE A and CIDE B (for cell death inducing DFF like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE A is a DFF45 inhibitable effector that promotes cell death and DNA fragmentation. CIDE A is expressed in many tissues. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptide- QAKGRFTCG |
| Positive Control | IHC - formalin-fixed, paraffin-embedded multi tumour tissue microarray |
| Subclass | IgG1 kappa |
| Myeloma Used | P3X63Ag8.653 |
| Strain | Balb/c |
| Notes | For IHC antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0 |
| Research Area | Apoptosis and Programmed Cell Death, Cancer, Cardiovascular, Cell Cycle, Epigenetics & Nuclear Signalling, Metabolism, Protein Degradation |
References: 2 entries
The expression of brown fat-associated proteins in colorectal cancer and the relationship of uncoupling protein 1 with prognosis.
Europe PMC ID: 30737786
The expression of brown fat associated proteins in colorectal cancer and the relationship of uncoupling protein 1 with prognosis. Alnabulsi et al. 2019. Int J Cancer. :. PMID: 30737786.
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References: 2 entries
The expression of brown fat-associated proteins in colorectal cancer and the relationship of uncoupling protein 1 with prognosis.
The expression of brown fat associated proteins in colorectal cancer and the relationship of uncoupling protein 1 with prognosis. Alnabulsi et al. 2019. Int J Cancer. :. PMID: 30737786.
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