CHO ICAM-1Fc Cell Line
Invented by Prof Nancy Hogg from Cancer Research UK London Research Institute: Lincoln's Inn Fields
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (10)
- Inventor Info
Info
| Catalogue Number | 152756 |
| Antigen/Gene or Protein Targets | Human ICAM-1Fc chimeric protein (five extracellular domains of ICAM-1 plus hinge, CH2 and CH3 domains of IgG1) |
| Parental Line | Chinese Hamster Ovary (CHO) |
| Host | Hamster |
| Tissue | Ovary |
| Model | Transgenic |
| Relevance | CHO ICAM-1Fc cells secrete a dimeric human ICAM-1Fc protein. ICAM-1 (CD54) is a major ligand for leukocyte CD11/CD18 integrins and used in adhesion assays to evaluate integrin activity. |
| Production Details | The chimeric wild-type ICAM-1Fc protein consists of the five extracellular domains of ICAM-1 joined to the hinge, CH2 and CH3 domains of IgG1. CHO cells were transfected using DEAE-dextran and the secreted proteins were collected in serum-free medium for 4 days. The proteins were purified on a Protein A–Sepharose affinity column using standard methods. |
| Conditional | No |
| Research Area | Adhesion, Immunology |
| Cellosaurus ID | CVCL_AS85 |
References: 10 entries
Stanley et al. 2008. EMBO J. 27(1):62-75. PMID: 18079697.
Intermediate-affinity LFA-1 binds alpha-actinin-1 to control migration at the leading edge of the T cell.
Europe PMC ID: 18079697
Smith et al. 2005. J Cell Biol. 170(1):141-51. PMID: 15983060.
A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes.
Europe PMC ID: 15983060
Smith et al. 2003. J Cell Sci. 116(Pt 15):3123-33. PMID: 12799414.
LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent detachment.
Europe PMC ID: 12799414
Stanley et al. 2000. Biochem J. 351(Pt 1):79-86. PMID: 10998349.
The second domain of intercellular adhesion molecule-1 (ICAM-1) maintains the structural integrity of the leucocyte function-associated antigen-1 (LFA-1) ligand-binding site in the first domain.
Europe PMC ID: 10998349
Stanley et al. 1998. J Biol Chem. 273(6):3358-62. PMID: 9452454.
The I domain of integrin LFA-1 interacts with ICAM-1 domain 1 at residue Glu-34 but not Gln-73.
Europe PMC ID: 9452454
Add a reference
References: 10 entries
Stanley et al. 2008. EMBO J. 27(1):62-75. PMID: 18079697.
Intermediate-affinity LFA-1 binds alpha-actinin-1 to control migration at the leading edge of the T cell.
Smith et al. 2005. J Cell Biol. 170(1):141-51. PMID: 15983060.
A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes.
Smith et al. 2003. J Cell Sci. 116(Pt 15):3123-33. PMID: 12799414.
LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent detachment.
Stanley et al. 2000. Biochem J. 351(Pt 1):79-86. PMID: 10998349.
The second domain of intercellular adhesion molecule-1 (ICAM-1) maintains the structural integrity of the leucocyte function-associated antigen-1 (LFA-1) ligand-binding site in the first domain.
Stanley et al. 1998. J Biol Chem. 273(6):3358-62. PMID: 9452454.
The I domain of integrin LFA-1 interacts with ICAM-1 domain 1 at residue Glu-34 but not Gln-73.
Add a reference
