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Catalogue Number 152756
Antigen/Gene or Protein Targets Human ICAM-1Fc chimeric protein (five extracellular domains of ICAM-1 plus hinge, CH2 and CH3 domains of IgG1)
Parental Line Chinese Hamster Ovary (CHO)
Host Hamster
Tissue Ovary
Model Transgenic
Relevance CHO ICAM-1Fc cells secrete a dimeric human ICAM-1Fc protein. ICAM-1 (CD54) is a major ligand for leukocyte CD11/CD18 integrins and used in adhesion assays to evaluate integrin activity.
Production Details The chimeric wild-type ICAM-1Fc protein consists of the five extracellular domains of ICAM-1 joined to the hinge, CH2 and CH3 domains of IgG1. CHO cells were transfected using DEAE-dextran and the secreted proteins were collected in serum-free medium for 4 days. The proteins were purified on a Protein A–Sepharose affinity column using standard methods.
Conditional No
Research Area Adhesion, Immunology
Cellosaurus ID CVCL_AS85

References: 10 entries

Stanley et al. 2008. EMBO J. 27(1):62-75. PMID: 18079697.

Intermediate-affinity LFA-1 binds alpha-actinin-1 to control migration at the leading edge of the T cell.

Europe PMC ID: 18079697

Smith et al. 2005. J Cell Biol. 170(1):141-51. PMID: 15983060.

A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes.

Europe PMC ID: 15983060

Smith et al. 2003. J Cell Sci. 116(Pt 15):3123-33. PMID: 12799414.

LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent detachment.

Europe PMC ID: 12799414

Stanley et al. 2000. Biochem J. 351(Pt 1):79-86. PMID: 10998349.

The second domain of intercellular adhesion molecule-1 (ICAM-1) maintains the structural integrity of the leucocyte function-associated antigen-1 (LFA-1) ligand-binding site in the first domain.

Europe PMC ID: 10998349

Stanley et al. 1998. J Biol Chem. 273(6):3358-62. PMID: 9452454.

The I domain of integrin LFA-1 interacts with ICAM-1 domain 1 at residue Glu-34 but not Gln-73.

Europe PMC ID: 9452454


Add a reference

References: 10 entries

Stanley et al. 2008. EMBO J. 27(1):62-75. PMID: 18079697.

Intermediate-affinity LFA-1 binds alpha-actinin-1 to control migration at the leading edge of the T cell.

Europe PMC ID: 18079697

Smith et al. 2005. J Cell Biol. 170(1):141-51. PMID: 15983060.

A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes.

Europe PMC ID: 15983060

Smith et al. 2003. J Cell Sci. 116(Pt 15):3123-33. PMID: 12799414.

LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent detachment.

Europe PMC ID: 12799414

Stanley et al. 2000. Biochem J. 351(Pt 1):79-86. PMID: 10998349.

The second domain of intercellular adhesion molecule-1 (ICAM-1) maintains the structural integrity of the leucocyte function-associated antigen-1 (LFA-1) ligand-binding site in the first domain.

Europe PMC ID: 10998349

Stanley et al. 1998. J Biol Chem. 273(6):3358-62. PMID: 9452454.

The I domain of integrin LFA-1 interacts with ICAM-1 domain 1 at residue Glu-34 but not Gln-73.

Europe PMC ID: 9452454


Add a reference

References: 10 entries

Stanley et al. 2008. EMBO J. 27(1):62-75. PMID: 18079697.

Intermediate-affinity LFA-1 binds alpha-actinin-1 to control migration at the leading edge of the T cell.

Smith et al. 2005. J Cell Biol. 170(1):141-51. PMID: 15983060.

A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes.

Smith et al. 2003. J Cell Sci. 116(Pt 15):3123-33. PMID: 12799414.

LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent detachment.

Stanley et al. 2000. Biochem J. 351(Pt 1):79-86. PMID: 10998349.

The second domain of intercellular adhesion molecule-1 (ICAM-1) maintains the structural integrity of the leucocyte function-associated antigen-1 (LFA-1) ligand-binding site in the first domain.

Stanley et al. 1998. J Biol Chem. 273(6):3358-62. PMID: 9452454.

The I domain of integrin LFA-1 interacts with ICAM-1 domain 1 at residue Glu-34 but not Gln-73.


Add a reference


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