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Catalogue Number 152756
Antigen/Gene or Protein Targets Human ICAM-1Fc chimeric protein (five extracellular domains of ICAM-1 plus hinge, CH2 and CH3 domains of IgG1)
Parental Line Chinese Hamster Ovary (CHO)
Host Hamster
Tissue Ovary
Model Transgenic
Relevance CHO ICAM-1Fc cells secrete a dimeric human ICAM-1Fc protein. ICAM-1 (CD54) is a major ligand for leukocyte CD11/CD18 integrins and used in adhesion assays to evaluate integrin activity.
Production Details The chimeric wild-type ICAM-1Fc protein consists of the five extracellular domains of ICAM-1 joined to the hinge, CH2 and CH3 domains of IgG1. CHO cells were transfected using DEAE-dextran and the secreted proteins were collected in serum-free medium for 4 days. The proteins were purified on a Protein A–Sepharose affinity column using standard methods.
Conditional No
Research Area Adhesion, Immunology
Cellosaurus ID CVCL_AS85

References: 5 entries

Stanley et al. 2008. EMBO J. 27(1):62-75. PMID: 18079697.

Smith et al. 2005. J Cell Biol. 170(1):141-51. PMID: 15983060.

Smith et al. 2003. J Cell Sci. 116(Pt 15):3123-33. PMID: 12799414.

Stanley et al. 2000. Biochem J. 351(Pt 1):79-86. PMID: 10998349.

Stanley et al. 1998. J Biol Chem. 273(6):3358-62. PMID: 9452454.


Add a reference

References: 5 entries

Stanley et al. 2008. EMBO J. 27(1):62-75. PMID: 18079697.

Smith et al. 2005. J Cell Biol. 170(1):141-51. PMID: 15983060.

Smith et al. 2003. J Cell Sci. 116(Pt 15):3123-33. PMID: 12799414.

Stanley et al. 2000. Biochem J. 351(Pt 1):79-86. PMID: 10998349.

Stanley et al. 1998. J Biol Chem. 273(6):3358-62. PMID: 9452454.


Add a reference

References: 5 entries

Stanley et al. 2008. EMBO J. 27(1):62-75. PMID: 18079697.

Smith et al. 2005. J Cell Biol. 170(1):141-51. PMID: 15983060.

Smith et al. 2003. J Cell Sci. 116(Pt 15):3123-33. PMID: 12799414.

Stanley et al. 2000. Biochem J. 351(Pt 1):79-86. PMID: 10998349.

Stanley et al. 1998. J Biol Chem. 273(6):3358-62. PMID: 9452454.


Add a reference


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