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Anti-PAT4 [PAT-4/9/H10]
RECENTLY UPDATED

Invented by Helen Turley from University of Oxford , Prof Deborah Goberdhan from University of Oxford
Invented at University of Oxford

Info

Catalogue Number 152743
Applications IHC WB
Antigen/Gene or Protein Targets PAT4 (SLC36A4)
Reactivity Human
Relevance The Anti-PAT4 [PAT-4/9/H10] antibody is a highly specific mouse monoclonal antibody against PAT4. Members of the proton-assisted amino-acid transporter (PAT) or solute-linked carrier 36 (SLC36) family have been identified as positive regulators of growth and mTORC1 signalling in flies.These effects were shown to be conserved by characterisation of the two ubiquitously transcribed human PATs, PAT1 (SLC36A1) and PAT4 (SLC36A4). PAT4 is upregulated in aggressive forms of colorectal cancer and a possible biomarker.
Host Mouse
Immunogen Antigenic amino acid sequence within the N-terminus of PAT4 (REELDMDVMRPLINE-C).
Positive Control 786-O human renal cancer cells
Subclass IgG2a kappa
Molecular Weight (kDa) ~60
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 10% FCS plus HAT essential
Notes A monoclonal antibody against PAT4 was created by immunising mice with a keyhole limpet haemocyanin-conjugated, cysteine-coupled peptide (Severn Biotech Ltd., Kidderminster, UK) based on an antigenic amino-acid sequence within the N terminus of PAT4 (REELDMDVMRPLINE-C).
Research Area Cell Signaling & Signal Transduction, Metabolism

References

There are 2 reference entries for this reagent.

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References: 2 entries

Fan et al. 2015. Oncogene. :. PMID: 26434594.

PAT4 levels control amino-acid sensitivity of rapamycin-resistant mTORC1 from the Golgi and affect clinical outcome in colorectal cancer.

Europe PMC ID: 26434594


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References: 2 entries

Fan et al. 2015. Oncogene. :. PMID: 26434594.

PAT4 levels control amino-acid sensitivity of rapamycin-resistant mTORC1 from the Golgi and affect clinical outcome in colorectal cancer.


Add a reference