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|Backbone Size (bp)||pET24b - 5310bp; pACpET24 - 3262bp|
|Vector Type||CAPN2/pET & CAPNS1deltaG1/pACpET (co-expressed)|
|Antigen/Gene or Protein Targets||Calpain-2 (m-Calpain), Calpain 2, Calpain2|
Calpain belongs to the superfamily of Ca2+-regulated cysteine proteases, which are indispensable to the regulation of various cellular functions. Of the 15 mammalian calpain isoforms, μ- and m-calpains are the best characterized. Both μ- and m-calpain are ubiquitously expressed and exist as heterodimers, containing a distinct 80-kDa catalytic subunit (CAPN1 and CAPN2, respectively) and the common, 30-kDa regulatory subunit (CAPNS1).
Calpain-2 (m-Calpain) Plasmid is an E.coli expression system for recombinant human m-calpain. As detailed in Hata et al., 2012 this is achieved by co-expressing CAPN2 with a C-terminal histidine tag (CAPN2/pET) and CAPNS1 lacking the first Gly-repeated region at the N-terminus (CAPNS1deltaG1/pACpET) in the BL21(DE3) strain. From 1L of E.coli culture, ~5mg of m-calpain was purified by three successive column chromatographies. Resulting proteins are stable for several months.
|Research Area||Cell Signaling & Signal Transduction|
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