Calpain-2 (m-Calpain) Vector
Invented by Dr Hiroyuki Sorimachi from Tokyo Metropolitan Institute Of Medical Science , Dr Shoji Hata from Tokyo Metropolitan Institute Of Medical Science
Invented at Tokyo Metropolitan Institute Of Medical Science
- Datasheet
- References (2)
- Inventor Info
Info
| Backbone Size (bp) | pET24b - 5310bp; pACpET24 - 3262bp |
| Vector Type | CAPN2/pET & CAPNS1deltaG1/pACpET (co-expressed) |
| Antigen/Gene or Protein Targets | Calpain-2 (m-Calpain), Calpain 2, Calpain2 |
| Relevance |
Calpain belongs to the superfamily of Ca2+-regulated cysteine proteases, which are indispensable to the regulation of various cellular functions. Of the 15 mammalian calpain isoforms, μ- and m-calpains are the best characterized. Both μ- and m-calpain are ubiquitously expressed and exist as heterodimers, containing a distinct 80-kDa catalytic subunit (CAPN1 and CAPN2, respectively) and the common, 30-kDa regulatory subunit (CAPNS1). Calpain-2 (m-Calpain) Plasmid is an E.coli expression system for recombinant human m-calpain. As detailed in Hata et al., 2012 this is achieved by co-expressing CAPN2 with a C-terminal histidine tag (CAPN2/pET) and CAPNS1 lacking the first Gly-repeated region at the N-terminus (CAPNS1deltaG1/pACpET) in the BL21(DE3) strain. From 1L of E.coli culture, ~5mg of m-calpain was purified by three successive column chromatographies. Resulting proteins are stable for several months. |
| Research Area | Cell Signaling & Signal Transduction |
References: 2 entries
Hata et al. 2012. J Biochem. 151(4):417-22. PMID: 22232565.
Efficient expression and purification of recombinant human m-calpain using an Escherichia coli expression system at low temperature.
Europe PMC ID: 22232565
Add a reference
References: 2 entries
Hata et al. 2012. J Biochem. 151(4):417-22. PMID: 22232565.
Efficient expression and purification of recombinant human m-calpain using an Escherichia coli expression system at low temperature.
Add a reference
