Rock2 (Cow):ER GFP Vector
Invented by Prof Michael Olson at Cancer Research UK Glasgow: The Beatson Institute
Rock2 (Cow):ER-KD GFP Vector
Invented by Prof Michael Olson at Cancer Research UK Glasgow: The Beatson Institute
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 152727 |
| Backbone Size (bp) | 5169 |
| Bacterial Resistance | Puromycin |
| Vector Type | pBabe-puro3 |
| Antigen/Gene or Protein Targets | Rock2, RockII |
| Relevance |
Rock2 (Cow):ER-KD Plasmid contains the kinase dead form of the catalytic domain of bovine Rock-II where lysine 121 in the ATP‐binding pocket has been changed to glycine (K121G). The dead kinase is fused at the carboxy-terminal end to the hormone‐binding domain of the mouse estrogen receptor (hbER) and enhanced green fluorescent protein (EGFP) at the amino terminus. Rock2 (Cow):ER GFP Plasmid facilitates the study of Rock signaling in a more regulated manner by offering a conditionally active form of Rock2 through its fusion to the hormone‐binding domain of the estrogen receptor (ER). Activation can be achieved using the estrogen antagonist 4‐hydroxytamoxifen (4‐HT). Ultimately, Rock2 activation leads to phosphorylation of proteins involved in the regulation of the actin‐myosin cytoskeleton, generation of pronounced actin stress fibers, and changes in cell morphology. Rock2 (Cow):ER GFP serves as a good complement for small‐molecule Rock2 inhibitors. Rock2 (Cow):ER-KD serves as a negative control for Rock2 (Cow):ER GFP Plasmid (catalogue code 152726). |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Cell Structure and Motility |
References: 2 entries
Croft et al. 2006. Methods Enzymol. 406:541-53. PMID: 16472686.
Conditional regulation of a ROCK-estrogen receptor fusion protein.
Europe PMC ID: 16472686
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References: 2 entries
Croft et al. 2006. Methods Enzymol. 406:541-53. PMID: 16472686.
Conditional regulation of a ROCK-estrogen receptor fusion protein.
Add a reference
