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Rock2 (Cow):ER-KD GFP Vector

Invented by Prof Michael Olson from Cancer Research UK Glasgow: The Beatson Institute
Invented at Cancer Research UK Glasgow: The Beatson Institute
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Schematic representation of Rock2 and the conditionally regulated Rock (Cow):ER showing the functional domains; PH, Pleckstrin homology domain, CRT, cysteine-rich domain. Amino acids 5-553 were joined in frame to eGFP and estrogen receptor hormone binding domain (hbER) moieties to create the Rock (Cow):ER fusion protein. A kinase dead version (Rock2 (Cow):ER-KD) was created by changing lysine 121 to glycine (K121G).
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Image thumbnail for Rock2 (Cow):ER-KD GFP Vector
Catalogue Number 152727
Backbone Size (bp) 5169
Bacterial Resistance Puromycin
Vector Type pBabe-puro3
Antigen/Gene or Protein Targets Rock2, RockII
Relevance Rock2 (Cow):ER-KD Plasmid contains the kinase dead form of the catalytic domain of bovine Rock-II where lysine 121 in the ATP‐binding pocket has been changed to glycine (K121G). The dead kinase is fused at the carboxy-terminal end to the hormone‐binding domain of the mouse estrogen receptor (hbER) and enhanced green fluorescent protein (EGFP) at the amino terminus.

Rock2 (Cow):ER GFP Plasmid facilitates the study of Rock signaling in a more regulated manner by offering a conditionally active form of Rock2 through its fusion to the hormone‐binding domain of the estrogen receptor (ER). Activation can be achieved using the estrogen antagonist 4‐hydroxytamoxifen (4‐HT). Ultimately, Rock2 activation leads to phosphorylation of proteins involved in the regulation of the actin‐myosin cytoskeleton, generation of pronounced actin stress fibers, and changes in cell morphology. Rock2 (Cow):ER GFP serves as a good complement for small‐molecule Rock2 inhibitors.

Rock2 (Cow):ER-KD serves as a negative control for Rock2 (Cow):ER GFP Plasmid (catalogue code 152726).
Research Area Cancer, Cell Signaling & Signal Transduction, Cell Structure and Motility

References

There are 2 reference entries for this reagent.

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References: 2 entries

Croft et al. 2006. Methods Enzymol. 406:541-53. PMID: 16472686.

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Conditional regulation of a ROCK-estrogen receptor fusion protein.

Europe PMC ID: 16472686

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References: 2 entries

Croft et al. 2006. Methods Enzymol. 406:541-53. PMID: 16472686.

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Conditional regulation of a ROCK-estrogen receptor fusion protein.

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