Anti-Collagen Type VII [LH7.2]
Invented by Prof Irene Leigh from Queen Mary University of London
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (8)
- Inventor Info
Info
| Catalogue Number | 152691 |
| Applications | ELISA IHC IF WB |
| Antigen/Gene or Protein Targets | Collagen Type VII |
| Reactivity | Human |
| Relevance |
Type VII Collagen is found in anchoring fibrils at the dermal-epidermal junction. Type VII collagen is defective in Recessive Dystrophic Epidemolysis Bullosa (RDEB). LH7.2 can be used for differentiating invasive from non-invasive melanoma through clear visualisation of appearance and integrity of epidermal basement membrane and diagnosis and antenatal diagnosis of RDEB. LH 7:2 recognizes an EBM antigen which may be important in the pathogenesis of RDEB. |
| Host | Mouse |
| Immunogen | Cells from a single cell suspension of epidermal cells (obtained from fresh human neonatal foreskin) were lysed with Nonidet P40 in phosphate buffered saline and the insoluble pellet was sonicated to prepare insoluble fractions. The epitope is on the NC1 domain. |
| Subclass | IgG1 kappa |
| Molecular Weight (kDa) | 250 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Research Area | Cancer, Cell Type or Organelle Marker |
References: 8 entries
Eldardiri et al. 2012. Tissue Eng Part A. 18(5-6):587-97. PMID: 21939396.
Wound contraction is significantly reduced by the use of microcarriers to deliver keratinocytes and fibroblasts in an in vivo pig model of wound repair and regeneration.
Europe PMC ID: 21939396
Al-Refu et al. 2011. Clin Exp Dermatol. 36(1):63-8. PMID: 20637030.
Immunohistochemistry of ultrastructural changes in scarring lupus erythematosus.
Europe PMC ID: 20637030
Watson et al. 2001. J Invest Dermatol. 116(5):672-8. PMID: 11348454.
A short-term screening protocol, using fibrillin-1 as a reporter molecule, for photoaging repair agents.
Europe PMC ID: 11348454
Shimizu et al. 1990. Br J Dermatol. 122(5):577-85. PMID: 2354110.
Epidermolysis bullosa acquisita antigen and the carboxy terminus of type VII collagen have a common immunolocalization to anchoring fibrils and lamina densa of basement membrane.
Europe PMC ID: 2354110
Add a reference
References: 8 entries
Eldardiri et al. 2012. Tissue Eng Part A. 18(5-6):587-97. PMID: 21939396.
Wound contraction is significantly reduced by the use of microcarriers to deliver keratinocytes and fibroblasts in an in vivo pig model of wound repair and regeneration.
Al-Refu et al. 2011. Clin Exp Dermatol. 36(1):63-8. PMID: 20637030.
Immunohistochemistry of ultrastructural changes in scarring lupus erythematosus.
Watson et al. 2001. J Invest Dermatol. 116(5):672-8. PMID: 11348454.
A short-term screening protocol, using fibrillin-1 as a reporter molecule, for photoaging repair agents.
Shimizu et al. 1990. Br J Dermatol. 122(5):577-85. PMID: 2354110.
Epidermolysis bullosa acquisita antigen and the carboxy terminus of type VII collagen have a common immunolocalization to anchoring fibrils and lamina densa of basement membrane.
Add a reference
