Anti-PLAP [H17E2]
Invented by Prof Walter Bodmer from University of Oxford
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (12)
- Inventor Info
Info
| Catalogue Number | 152689 |
| Applications | ELISA IHC |
| Antigen/Gene or Protein Targets | Placenta-specific isozyme of Alkaline Phosphatase (PLAP) |
| Reactivity | Human |
| Relevance |
Monoclonal antibody detects PLAP protein, used as a marker for various cancers, including ovarian and testicular. Background and Research Application Human placental alkaline phosphatase (PLAP) is a membrane bound glycosylated phosphodiesterase normally synthesised by syncytiotrophoblast from the 12th week of pregnancy. Since its identification as an oncofoetal antigen, it has been found to be expressed by malignant tumours of germ cell and non-germ cell origin. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. The placental-specific isozyme of Alkaline Phosphatase (PLAP), also referred to as the heat-stable form, is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. It is closely related to the intestinal form of the enzyme as well as to the placental-like form. Anti-PLAP was created for use in immunoscintigraphy or therapy. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. The antibody detects both Regan and Nagao isoenzymes. |
| Host | Mouse |
| Immunogen | Placental membranes prepared from fresh term placenta |
| Immunogen UniProt ID | P05187 |
| Subclass | IgG1 |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Anti-PLAP has a lack of reactivity with normal tissues, rendering it potentially useful as a therapeutic agent in the treatment of testicular germ cell tumours. All seminomas and malignant teratomas tested gave strong positive labelling with H17E2. Concentration 1mg/ml as standard |
| Research Area | Cancer, Cell Type or Organelle Marker |
References: 12 entries
Fitzgerald et al. 2018. Am J Reprod Immunol. 80(1):e12860. PMID: 29726582.
Streicher et al. 2017. Sci Rep. 7(1):6460. PMID: 28744019.
Multicolor flow cytometry and nanoparticle tracking analysis of extracellular vesicles in the plasma of normal pregnant and pre-eclamptic women.
Europe PMC ID: 24227753
Dragovic et al. 2013. Biol Reprod. 89(6):151. PMID: 24227753.
Tucker et al. 1985. Br J Cancer. 51(5):631-9. PMID: 2986664.
Epenetos et al. 1985. Br J Cancer. 51(5):641-4. PMID: 3994908.
Serum marker potential of placental alkaline phosphatase-like activity in testicular germ cell tumours evaluated by H17E2 monoclonal antibody assay.
Europe PMC ID: 2986664
Monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours.
Europe PMC ID: 3994908
Travers et al. 1984. Int J Cancer. 33(5):633-41. PMID: 6202646.
Preparation and characterization of monoclonal antibodies against placental alkaline phosphatase and other human trophoblast-associated determinants.
Europe PMC ID: 6202646
Epenetos et al. 1984. Br J Cancer. 49(1):11-5. PMID: 6362705.
An immunohistological study of testicular germ cell tumours using two different monoclonal antibodies against placental alkaline phosphatase.
Europe PMC ID: 6362705
Add a reference
References: 12 entries
Fitzgerald et al. 2018. Am J Reprod Immunol. 80(1):e12860. PMID: 29726582.
Streicher et al. 2017. Sci Rep. 7(1):6460. PMID: 28744019.
Multicolor flow cytometry and nanoparticle tracking analysis of extracellular vesicles in the plasma of normal pregnant and pre-eclamptic women.
Dragovic et al. 2013. Biol Reprod. 89(6):151. PMID: 24227753.
Tucker et al. 1985. Br J Cancer. 51(5):631-9. PMID: 2986664.
Epenetos et al. 1985. Br J Cancer. 51(5):641-4. PMID: 3994908.
Serum marker potential of placental alkaline phosphatase-like activity in testicular germ cell tumours evaluated by H17E2 monoclonal antibody assay.
Monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours.
Travers et al. 1984. Int J Cancer. 33(5):633-41. PMID: 6202646.
Preparation and characterization of monoclonal antibodies against placental alkaline phosphatase and other human trophoblast-associated determinants.
Epenetos et al. 1984. Br J Cancer. 49(1):11-5. PMID: 6362705.
An immunohistological study of testicular germ cell tumours using two different monoclonal antibodies against placental alkaline phosphatase.
Add a reference
