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HRN™ Mouse

Invented by Prof Roland Wolf at University of Dundee

Info

Catalogue Number 152669
Antigen/Gene or Protein Targets Cytochrome P450 (POR)
Relevance The HRN™ mouse model lacks P450 activity in the liver and can be used to study the role of hepatic P450 in drug metabolism and to determine the efficacy or toxicity of pharmacological compounds. HRN™ mice are homozygous for the floxed Por allele and the Alb-Cre transgene. POR is the electron donating enzyme for all of cytochrome P450 enzymes.
Production Details The HRN™ model was created by targeting the Por gene to generate a floxed allele in GK129/1 embryonic stem cells derived from 129P2 mice and injecting the targeted cells into C57BL/6 blastocysts. Resultant chimeras were backcrossed to C57BL/6 for one generation. Mice heterozygous for the floxed Por allele were intercrossed to generate mice homozygous for the floxed Por allele on a mixed B6;129P2 background. The Alb-cre transgene was developed in the laboratory of Mark A. Magnuson at Vanderbilt University School of Medicine by microinjecting Cre recombinase gene under the control of the rat albumin enhancer/promoter into B6D2F2 zygotes. Mice homozygous for the floxed Por allele were bred to carriers for the Alb-cre transgene to generate HRN™ mice.
Conditional Yes
Conditional Description The Por gene is flanked by loxP sites. Cre recombinase is expressed under the rat Albumin enhancer/promoter. The mice exhibit liver-specific conditional deletion of POR, and subsequent disruption of the hepatic P450 metabolism.
Strain C57BL/6
Mouse Genetic Background/Cross History The HRN™ model was backcrossed to C57BL/6J a total of 6 generations (N6).
Zygosity Homozygous
Research Area Cancer, Drug Discovery & Development, Genetic Studies Tools, Metabolism
Notes HRN stands for Hepatic Cytochrome P450 Reductase Null

The publication refers to the HRN™ parent strain

References: 1 entry

Henderson et al. 2003. J Biol Chem. 278(15):13480-6. PMID: 12566435.


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References: 1 entry

Henderson et al. 2003. J Biol Chem. 278(15):13480-6. PMID: 12566435.


Add a reference

References: 1 entry

Henderson et al. 2003. J Biol Chem. 278(15):13480-6. PMID: 12566435.


Add a reference


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