Anti-NS5B [1A4]
Invented at A*STAR Accelerate Technologies Pte Ltd
- Datasheet
- References (2)
- Inventor Info
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![Western blotting using full length NS5B proteins (with a N-terminal flag-tag) from 3 HCV genotypes; 1b, 2a, and 3a. Mock refers to cells transfected with empty vector only. Detection was performed using anti-NS5B [1A4] (at a concentration of 2μg/ml) or an anti-FLAG antibody.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/c9ed651a-7f2c-4e0e-96c3-26d9bbfb157c.png)
![Western blotting on lysates prepared from Huh7.5 cells infected or mock infected with HCV using anti-NS5B [1A4]. An antibody against β-actin was used as loading control.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/30e95aff-781f-4270-bacb-7eef3bdeffae.png)
![Immunofluorescence on Huh7.5 cells mock infected or infected with HCV using anti-NS5B [1A4]. Cells were fixed using 4% formaldehyde solution at 2 days post infection. The antibody was applied at a concentration of 20μg/ml. DAPI was used to stain the nucleus.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/eae946c4-b570-4203-a749-c7b7e04a1da8.png)
![Western blotting using full length NS5B proteins (with a N-terminal flag-tag) from 3 HCV genotypes; 1b, 2a, and 3a. Mock refers to cells transfected with empty vector only. Detection was performed using anti-NS5B [1A4] (at a concentration of 2μg/ml) or an anti-FLAG antibody.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/c9ed651a-7f2c-4e0e-96c3-26d9bbfb157c.png)
![Western blotting on lysates prepared from Huh7.5 cells infected or mock infected with HCV using anti-NS5B [1A4]. An antibody against β-actin was used as loading control.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/30e95aff-781f-4270-bacb-7eef3bdeffae.png)
![Immunofluorescence on Huh7.5 cells mock infected or infected with HCV using anti-NS5B [1A4]. Cells were fixed using 4% formaldehyde solution at 2 days post infection. The antibody was applied at a concentration of 20μg/ml. DAPI was used to stain the nucleus.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/eae946c4-b570-4203-a749-c7b7e04a1da8.png)
![Image thumbnail for Anti-NS5B [1A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/c9ed651a-7f2c-4e0e-96c3-26d9bbfb157c.png)
![Image thumbnail for Anti-NS5B [1A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/30e95aff-781f-4270-bacb-7eef3bdeffae.png)
![Image thumbnail for Anti-NS5B [1A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/eae946c4-b570-4203-a749-c7b7e04a1da8.png)
| Catalogue Number | 152664 |
| Applications | IF WB |
| Antigen/Gene or Protein Targets | Hepatitis C virus non-structural protein 5B |
| Reactivity | Virus |
| Relevance | Hepatitis C virus (HCV) encodes a polyprotein that is cleaved into at least 10 viral proteins namely E1, E2, core, p7, NS2, NS3, NS4A, NS4B, NS5A, and NS5B. Non-structural 5B protein (NS5B) is HCV’s RNA dependant RNA polymerase and is critical for viral replication. |
| Host | Mouse |
| Immunogen | GST-NS5B (residues 1-346) fusion protein of a HCV genotype 2a strain |
| Subclass | IgG2a |
| Myeloma Used | Sp2/0-Ag14 |
| Research Area | Virology |
References: 2 entries
Aweya et al. 2015. Virology. 474:41-51. PMID: 25463603.
NS5B induces up-regulation of the BH3-only protein, BIK, essential for the hepatitis C virus RNA replication and viral release.
Europe PMC ID: 25463603
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References: 2 entries
Aweya et al. 2015. Virology. 474:41-51. PMID: 25463603.
NS5B induces up-regulation of the BH3-only protein, BIK, essential for the hepatitis C virus RNA replication and viral release.
Add a reference
