Anti-HA (H5N1) [9F4]
Invented at A*STAR Accelerate Technologies Pte Ltd
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- References (4)
- Inventor Info
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![FACS analysis on 293T cells transfected either with a cDNA construct expressing Hatay04 HA or with an empty vector. Cells were stained with anti-HA (H5N1) [9F4] followed by a FITC-conjugated anti-mouse IgG (open histograms) or with FITC-conjugated anti-mouse IgG only (shaded histograms).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/9f3da2fe-eaa6-43df-8cee-c3b3c2c19d06.png)
![Prophylactic and therapeutic protection against H5N1 virus by anti-HA (H5N1) [9F4]. (A and B) To test for prophylactic efficacy, BALB/c mice were immunized either with anti-HA (H5N1) [9F4] at different doses, with an irrelevant IgG control MAb (1 mg/kg), or with PBS and were subjected to viral challenge 24 h later. A group of mice was mock infected. Mice were observed daily for disease and mortality and were weighed every third day until they had to be euthanized or until day 10 after challenge. (C and D) To test for therapeutic efficacy, anti-HA (H5N1) [9F4] was administered 24 h after viral challenge, and the mice were monitored as described above.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/f06dc893-6899-4910-990d-c9c7360e4ff3.png)
![Immunofluorescence was performed on MDCK cells transfected with empty vector or DL06-HA or Hatay04-HA expressing plasmids using Anti-HA (H5N1) [9F4] on non-permeabilized cells. A secondary Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody was applied for detection purposes.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/f6b6aa5c-0d6f-446a-9663-55792306ce40.png)
![FACS analysis on 293T cells transfected either with a cDNA construct expressing Hatay04 HA or with an empty vector. Cells were stained with anti-HA (H5N1) [9F4] followed by a FITC-conjugated anti-mouse IgG (open histograms) or with FITC-conjugated anti-mouse IgG only (shaded histograms).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/9f3da2fe-eaa6-43df-8cee-c3b3c2c19d06.png)
![Prophylactic and therapeutic protection against H5N1 virus by anti-HA (H5N1) [9F4]. (A and B) To test for prophylactic efficacy, BALB/c mice were immunized either with anti-HA (H5N1) [9F4] at different doses, with an irrelevant IgG control MAb (1 mg/kg), or with PBS and were subjected to viral challenge 24 h later. A group of mice was mock infected. Mice were observed daily for disease and mortality and were weighed every third day until they had to be euthanized or until day 10 after challenge. (C and D) To test for therapeutic efficacy, anti-HA (H5N1) [9F4] was administered 24 h after viral challenge, and the mice were monitored as described above.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/f06dc893-6899-4910-990d-c9c7360e4ff3.png)
![Immunofluorescence was performed on MDCK cells transfected with empty vector or DL06-HA or Hatay04-HA expressing plasmids using Anti-HA (H5N1) [9F4] on non-permeabilized cells. A secondary Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody was applied for detection purposes.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/f6b6aa5c-0d6f-446a-9663-55792306ce40.png)
![Image thumbnail for Anti-HA (H5N1) [9F4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/9f3da2fe-eaa6-43df-8cee-c3b3c2c19d06.png)
![Image thumbnail for Anti-HA (H5N1) [9F4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/f06dc893-6899-4910-990d-c9c7360e4ff3.png)
![Image thumbnail for Anti-HA (H5N1) [9F4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/f6b6aa5c-0d6f-446a-9663-55792306ce40.png)
| Catalogue Number | 152661 |
| Applications | IF Fn WB |
| Antigen/Gene or Protein Targets | H5N1 Influenza A virus hemagglutinin protein |
| Reactivity | Virus |
| Relevance | The highly pathogenic avian influenza A subtype H5N1 virus was first isolated from geese in Guangdong province, China, in 1996. Since 2003, the H5N1 strains have caused major morbidity and mortality in poultry populations across Asia, Europe, and Africa The hemagglutinin (HA) protein is one of the two major surface glycoproteins on the envelope of influenza A virus, with 16 distinct types identified in the avian species. The HA protein is responsible for receptor binding to host cells and for viral entry and is therefore the primary target of neutralizing antibodies. In this study, the recombinant HA protein of a clade H5N1 virus was used to generate monoclonal antibodies for viral neutralization assays. The HA full-length protein was expressed in insect cells infected with baculovirus carrying the HA gene to ensure that it underwent glycosylation. |
| Host | Mouse |
| Immunogen | HA full length that was expressed and purified from insect cells |
| Positive Control | Transfected MDCK cells |
| Subclass | IgG2b |
| Myeloma Used | Sp2/0-Ag14 |
| Research Area | Virology |
References: 4 entries
Mak et al. 2014. Antiviral Res. 107:76-83. PMID: 24797696.
Fn
Chimerization and characterization of a monoclonal antibody with potent neutralizing activity across multiple influenza A H5N1 clades.
Europe PMC ID: 24797696
Oh et al. 2010. J Virol. 84(16):8275-86. PMID: 20519402.
Fn
An antibody against a novel and conserved epitope in the hemagglutinin 1 subunit neutralizes numerous H5N1 influenza viruses.
Europe PMC ID: 20519402
Add a reference
References: 4 entries
Mak et al. 2014. Antiviral Res. 107:76-83. PMID: 24797696.
Fn
Chimerization and characterization of a monoclonal antibody with potent neutralizing activity across multiple influenza A H5N1 clades.
Oh et al. 2010. J Virol. 84(16):8275-86. PMID: 20519402.
Fn
An antibody against a novel and conserved epitope in the hemagglutinin 1 subunit neutralizes numerous H5N1 influenza viruses.
Add a reference
