Anti-VTN4 [10H1]
Invented at A*STAR Accelerate Technologies Pte Ltd
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![Western blot was performed on 10ug of total cell lysate from Jurkat cells (lane 1), untransfected 293T cells (lane 2), 293T transfected with pIRES2-GFP backbone vector (lane 3) and with pIRES2-GFP-zebrafish NEMO (lane 4). Anti-VTN4 [10H1] was applied at a dilution of 1:10, 000](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/fb21842e-8f3d-473b-a12f-da4cb59cd11f.png)
![Western blot was performed on total cell lysates from transfected 293T cells (lane 1) or zebrafishes at different stages of development (lanes 3 to 10). Luminescence markers were loaded in lane 2. Anti-VTN4 [10H1] detects endogenous VTN4, with the protein level varying according to the development stages in zebrafish](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/6349b4fc-30d7-4092-ab02-6bdcc4f59de1.png)
![Immunofluorescence of early stage zebrafish embryos demonstrates Anti-VTN4 [10H1] can detect the endogenous target. The antibody was applied using a 1:500 dilution and the FITC-conjugated secondary antibody at a dilution of 1: 500](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/88c58c51-404b-4018-8a2a-c170ce6681a0.png)
![Western blot was performed on 10ug of total cell lysate from Jurkat cells (lane 1), untransfected 293T cells (lane 2), 293T transfected with pIRES2-GFP backbone vector (lane 3) and with pIRES2-GFP-zebrafish NEMO (lane 4). Anti-VTN4 [10H1] was applied at a dilution of 1:10, 000](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/fb21842e-8f3d-473b-a12f-da4cb59cd11f.png)
![Western blot was performed on total cell lysates from transfected 293T cells (lane 1) or zebrafishes at different stages of development (lanes 3 to 10). Luminescence markers were loaded in lane 2. Anti-VTN4 [10H1] detects endogenous VTN4, with the protein level varying according to the development stages in zebrafish](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/6349b4fc-30d7-4092-ab02-6bdcc4f59de1.png)
![Immunofluorescence of early stage zebrafish embryos demonstrates Anti-VTN4 [10H1] can detect the endogenous target. The antibody was applied using a 1:500 dilution and the FITC-conjugated secondary antibody at a dilution of 1: 500](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/88c58c51-404b-4018-8a2a-c170ce6681a0.png)
![Image thumbnail for Anti-VTN4 [10H1]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/fb21842e-8f3d-473b-a12f-da4cb59cd11f.png)
![Image thumbnail for Anti-VTN4 [10H1]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/6349b4fc-30d7-4092-ab02-6bdcc4f59de1.png)
![Image thumbnail for Anti-VTN4 [10H1]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/88c58c51-404b-4018-8a2a-c170ce6681a0.png)
| Catalogue Number | 152658 |
| Applications | IF WB |
| Antigen/Gene or Protein Targets | Full length protein of zebrafish IKKg (602 amino acids) |
| Reactivity | Zebrafish |
| Relevance | VTN4 is Zebrafish full length IKKγ. Through alignment studies (unpublished), the C-terminal end of IKKγ is conversed across species while the N-terminal is unique to Zebrafish. Thus, the generation of monoclonal antibodies to Zebrafish IKKγ is useful in developmental studies being carried out in Zebrafish model. |
| Host | Mouse |
| Immunogen | GST-VTN4 fusion protein |
| Subclass | IgG2b |
| Myeloma Used | Sp2/0-Ag14 |
| Research Area | Aquaculture, Developmental Biology |
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