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|Antigen/Gene or Protein Targets||Member of Vamp subfamily of SNAREs (Soluble NSF attachment protein receptors)|
SNAREs represent a superfamily of proteins responsible for the last stage of docking and subsequent fusion in diverse intracellular membrane transport events. The Vamp subfamily of SNAREs contains 7 members (Vamp1, Vamp2, Vamp3/cellubrevin, Vamp4, Vamp5, Vamp7/Ti-Vamp, and Vamp8/endobrevin) that are distributed in various post-Golgi structures. Vamp4 and Vamp5 are distributed predominantly in the trans-Golgi network (TGN) and the plasma membrane, respectively.When C-terminally tagged with enhanced green fluorescent protein, the majority of Vamp4 and Vamp5 is correctly
targeted to the TGN and plasma membrane, respectively. Swapping the N-terminal cytoplasmic region and the C-terminal membrane anchor domain between Vamp4 and Vamp5 demonstrates that the N-terminal cytoplasmic region of these two SNAREs contains the correct subcellular targeting information.
|Immunogen||GST-mV5-II and KLH-mV5-II peptide sequences|
|Research Area||Cell Signaling & Signal Transduction|
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