Anti-SΔ3 [7G12]
Invented at A*STAR Accelerate Technologies Pte Ltd
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- References (3)
- Inventor Info
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![Western blotting on 293T cells transfected with expression plasmid for wild-type S and mutated forms of S (S(N1056K), S(D1128A), S(N1056K, D1128A)) and mock transfected using anti-SΔ3 [7G12].](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/97b1c3de-bd81-474e-9916-5f4069007e57.png)
![Immunoprecipitation of radiolabelled wild-type S and mutated forms of S (S(N1056K), S(D1128A), S(N1056K, D1128A)) from transfected 293T cells using anti-SΔ3 [7G12]. A mock transfection was included as a control. Subsequent western blot was performed using the same antibody.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/3350467b-689e-4646-8b17-dca4f8d26f70.png)
![Western blotting on 293T cells transfected with expression plasmid for wild-type S and mutated forms of S (S(N1056K), S(D1128A), S(N1056K, D1128A)) and mock transfected using anti-SΔ3 [7G12].](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/97b1c3de-bd81-474e-9916-5f4069007e57.png)
![Immunoprecipitation of radiolabelled wild-type S and mutated forms of S (S(N1056K), S(D1128A), S(N1056K, D1128A)) from transfected 293T cells using anti-SΔ3 [7G12]. A mock transfection was included as a control. Subsequent western blot was performed using the same antibody.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/3350467b-689e-4646-8b17-dca4f8d26f70.png)
![Image thumbnail for Anti-SΔ3 [7G12]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/97b1c3de-bd81-474e-9916-5f4069007e57.png)
![Image thumbnail for Anti-SΔ3 [7G12]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/3350467b-689e-4646-8b17-dca4f8d26f70.png)
| Catalogue Number | 152651 |
| Applications | IP WB |
| Antigen/Gene or Protein Targets | S protein of the SARS coronavirus |
| Reactivity | Virus |
| Relevance |
Monoclonal antibody used to aid understanding the method of SARS membrane fusion and entry. Background and Research Application Severe acute respiratory syndrome coronavirus (SARS-CoV) is a species of coronavirus known to infect humans, bats, and certain other mammals. The SARS-related coronavirus is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by the ACE2 receptor. SARS genome encodes for four structural proteins, namely nucleocaspid (N), spike (S), membrane (M) and envelope (E). The S protein, which forms morphologically characteristic projections on the virion surface, is responsible for binding to cellular receptor and mediating the fusion between viral and cellular membranes. The SΔ3 is a fragment containing residues from 168 to 461 of the S protein. This antibody has use in investigating the mechanism of membrane fusion and virus entry. |
| Host | Mouse |
| Immunogen | GST-SΔ3 fusion protein |
| Subclass | IgG1 |
| Myeloma Used | Sp2/0-Ag14 |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest This antibody was found to have no viral neutralizing activity (Lip et al., 2006). Concentration 1mg/ml as standard |
| Research Area | Virology |
References: 3 entries
Lip et al. 2006. J Virol. 80(2):941-50. PMID: 16378996.
Lip et al. 2006. J Virol. 80(2):941-50. PMID: 16378996.
Monoclonal antibodies targeting the HR2 domain and the region immediately upstream of the HR2 of the S protein neutralize in vitro infection of severe acute respiratory syndrome coronavirus.
Europe PMC ID: 16378996
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References: 3 entries
Lip et al. 2006. J Virol. 80(2):941-50. PMID: 16378996.
Lip et al. 2006. J Virol. 80(2):941-50. PMID: 16378996.
Monoclonal antibodies targeting the HR2 domain and the region immediately upstream of the HR2 of the S protein neutralize in vitro infection of severe acute respiratory syndrome coronavirus.
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