Anti-BHMT [3E7]
Invented at A*STAR Accelerate Technologies Pte Ltd
- Datasheet
- References (2)
- Inventor Info
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![Western blot analysis using anti-BHMT [3E7] on Zebrafish embryo lysate (+) and control BHMT KO Zebrafish control lysate (-)](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/c6abb3e6-c5ec-44a4-b219-49e4212e96be.jpg)
![Immunofluorescence using anti-BHMT [3E7] on Zebrafish embryos. +ve and -ve controls correspond to the presence and absence of anti-BHMT [3E7] respectively.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/63ff0e79-9c11-4b26-aef7-e1650d2336a3.jpg)
![Western blot analysis using anti-BHMT [3E7] on Zebrafish embryo lysate (+) and control BHMT KO Zebrafish control lysate (-)](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/c6abb3e6-c5ec-44a4-b219-49e4212e96be.jpg)
![Immunofluorescence using anti-BHMT [3E7] on Zebrafish embryos. +ve and -ve controls correspond to the presence and absence of anti-BHMT [3E7] respectively.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/63ff0e79-9c11-4b26-aef7-e1650d2336a3.jpg)
![Image thumbnail for Anti-BHMT [3E7]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/c6abb3e6-c5ec-44a4-b219-49e4212e96be.jpg)
![Image thumbnail for Anti-BHMT [3E7]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/63ff0e79-9c11-4b26-aef7-e1650d2336a3.jpg)
| Catalogue Number | 152637 |
| Applications | FACS WB |
| Antigen/Gene or Protein Targets | Betaine homocysteine S-methyltransferase |
| Reactivity | Zebrafish |
| Relevance | Betaine homocysteine S-methyltransferase (BHMT) shares 61% sequence homology between human and mouse gene. This 406 amino acids protein is found in both eukaryotes and prokaryotes. Homocysteine is generated from S-adenosylhomocysteine (SAH) through S adenosylhomocysteine hydrolase (SAHH) catalyzed reaction, into homocysteine and adenosine. This reversible reaction is thermodynamically favoured in the reverse reaction, synthesizing SAH. Therefore, to prevent the reverse reaction, adenosine is metabolized by adenosine deaminase and homocysteine is metabolized through several means of which BHMT is one of them. The action of BHMT is to catalyze resynthesis of methionine using homocysteine and methyl donor betaine. Betaine could be obtained from diet and in mammalian cells, it can be produced by choline oxidation in liver and kidney. Studies in many animal models revealed that liver and kidney are main organs for BHMT. BHMT presence is also detected in lens as well as in hepatocyte membrane, which thus lends speculations about other possible physiological roles besides methionine synthesis. Therefore, much studies have to be undertaken, to understanding the other physiological roles, if any, of BHMT besides the traditional role in methylation in future. |
| Host | Mouse |
| Immunogen | GST-BHMT fusion protein |
| Positive Control | Zebrafish embryo lysate |
| Subclass | IgG1 |
| Myeloma Used | Sp2/0-Ag14 |
| Research Area | Aquaculture, Cancer, Cell Signaling & Signal Transduction, Metabolism |
References: 2 entries
Yang et al. 2011. Endocrinology. 152(12):4706-17. PMID: 21952238.
Depletion of Bhmt elevates sonic hedgehog transcript level and increases β-cell number in zebrafish.
Europe PMC ID: 21952238
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References: 2 entries
Yang et al. 2011. Endocrinology. 152(12):4706-17. PMID: 21952238.
Depletion of Bhmt elevates sonic hedgehog transcript level and increases β-cell number in zebrafish.
Add a reference
