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Monoclonal Antibodies

Anti-sCTLA4 [L4P2F5*F10]

Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
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Western blot was performed using Jurkat cell lysates. Two products are detected, slightly larger than anticipated-possibly due to glycosylation.
A. Immunohistochemical staining of formalin-fixed, paraffin-embedded human lymph node using anti-sCTLA44 [L4P2F5*F10]. Cytoplasmic staining is shown as expected (CTLA4 is expressed in T-cells). B. Positive cytoplasmic staining was also detected in human breast cancer sections.
Anti-sCTLA4 antibody [L4P2F5*F10] was applied to detect soluble CTLA4 in mouse serum by indirect ELISA.
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Catalogue Number 152604
Applications ELISA IHC WB
Antigen/Gene or Protein Targets Cytotoxic T-lymphocyte-associated protein 4, sCTLA4 molecule (sCTLA4 soluble form)
Synonyms Cytotoxic T-Lymphocyte Associated Protein 4; Insulin-Dependent Diabetes Mellitus 12; Celiac Disease 3; CTLA-4; CD152; CD152 Antigen; CELIAC3; IDDM12; ALPS5; GRD4; GSE; CD
Reactivity Bovine, Human, Mouse and Rat
Relevance CTLA4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein that plays an important regulatory role in the immune system. CTLA4 is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. Mutations in the CTLA4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases.

A recently identified novel transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD ) had a high concentration of sCTLA-4, whereas only 1 of 30 apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.

There is strong evidence showing variation of sCTLA4 levels between individuals ranging from approximately 150 to around 10,000 pg/ ml. One can speculate that those patients with high sCTLA4 levels are unlikely to benefit from CTLA4 therapy. Hence, it is crucial to measure/ quantify sCTLA4 before administering treatment. This anti-sCTLA4 antibody could prove a useful predictive biomarker.
Host Mouse
Immunogen Ovalbumin-conjugated synthetic peptide CENAPNRARM
Positive Control IHC- formalin-fixed, paraffin-embedded multi tumour tissue microarray; WB - Jurkat cell lysates
Subclass IgG1 kappa
Notes Immunogen sequence is identical to mouse (Mus musculus), rat (Rattus norvegicus) and bovine (Bos taurus).
Research Area Immunology, Stem Cell Biology

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