Anti-Cytochrome P450 46A1 [N8-P6E4*H8]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
- References (2)
- Inventor Info
Info
![Western blot was performed using anti-Cytochrome P450 46A1 [N8-P6E4*H8]. The left hand lane contains control cell lysate while the right hand lane contains lysate prepared from cells over-expressing the protein. ~15 micrograms of protein were loaded per well. No bands were detected with the negative control lysate, while a band migrating at the expected molecular weight was observed in the over-expression lysate.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/0f17bdf1-ada5-4111-98a8-9a655fa0ac4b.jpg)
![Western blot was performed using anti-Cytochrome P450 46A1 [N8-P6E4*H8]. The left hand lane contains control cell lysate while the right hand lane contains lysate prepared from cells over-expressing the protein. ~15 micrograms of protein were loaded per well. No bands were detected with the negative control lysate, while a band migrating at the expected molecular weight was observed in the over-expression lysate.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/0f17bdf1-ada5-4111-98a8-9a655fa0ac4b.jpg)
![Image thumbnail for Anti-Cytochrome P450 46A1 [N8-P6E4*H8]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/be05828a-2ac4-4cdf-ae66-4cc83abae39f.jpg)
![Image thumbnail for Anti-Cytochrome P450 46A1 [N8-P6E4*H8]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/0f17bdf1-ada5-4111-98a8-9a655fa0ac4b.jpg)
| Catalogue Number | 152600 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Cytochrome P450, Family 46, Subfamily A, Polypeptide 1 (CYP46A1) |
| Reactivity | Human |
| Relevance | Cytochrome P450 46A1 belongs to the cytochrome P450 family which are a group of monooxygenases. Cytochrome P450 46A1 is involved in the turnover of cholesterol converting it into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. This protein is expressed in the brain and mRNA has been found to be broadly distributed with higher levels in the gray matter zones and lower levels in white matter areas. It is not detected in fetal brain but its expression increases linearly with age. It localises to the endoplasmic reticulum membrane and the microsome membrane. The unprocessed precursor is 500 amino acids long and has a predicted molecular weight of 56.8KD. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptide- PVLCTLRPR (C-terminal sequence) |
| Positive Control | WB - overexpression lysates |
| Subclass | IgG1 |
| Molecular Weight (kDa) | 57 |
| Notes | This antibody was applied in a poster presented at the 2015 NCRI conference: http://abstracts.ncri.org.uk/?s=oxysterol&year_published=2015 |
| Research Area | Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Metabolism |
References: 2 entries
Characterisation of the oxysterol metabolising enzyme pathway in mismatch repair proficient and deficient colorectal cancer.
Europe PMC ID: 27341022
Swan et al. 2016. Oncotarget. :. PMID: 27341022.
IHC WB
Add a reference
References: 2 entries
Characterisation of the oxysterol metabolising enzyme pathway in mismatch repair proficient and deficient colorectal cancer.
Swan et al. 2016. Oncotarget. :. PMID: 27341022.
IHC WB
Add a reference
