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Anti-Cytochrome P450 27A1 [V29-P4B8*B2]

Invented by Ayham Alnabulsi at Vertebrate Antibodies Limited

Info

Catalogue Number 152599
Applications ELISA IHC WB
Antigen/Gene or Protein Targets Cytochrome P450, Family 27, Subfamily A, Polypeptide 1 (CYP27A1), Sterol 27-Hydroxylase
Reactivity Human
Relevance Cytochrome P450 27A1 (CYP27A1) catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.
Host Mouse
Immunogen Ovalbumin-conjugated synthetic peptide KVVLAPETGEL (C-terminal sequence)
Positive Control IHC: formalin-fixed, paraffin-embedded human sections; WB: Overexpression lysates
Subclass IgG1 kappa
Notes For IHC perform antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0

This antibody was applied in a poster presented at the 2015 NCRI conference: http://abstracts.ncri.org.uk/?s=oxysterol&year_published=2015
Research Area Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Metabolism

References

There are 2 reference entries for this reagent.

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References: 2 entries

Characterisation of the oxysterol metabolising enzyme pathway in mismatch repair proficient and deficient colorectal cancer.

Europe PMC ID: 27341022

Swan et al. 2016. Oncotarget. :. PMID: 27341022.

IHC WB


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References: 2 entries

Characterisation of the oxysterol metabolising enzyme pathway in mismatch repair proficient and deficient colorectal cancer.

Swan et al. 2016. Oncotarget. :. PMID: 27341022.

IHC WB


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