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Anti-VEGF, Recombinant [VG76e]

Invented by Prof Roy Bicknell at Absolute Antibody, University of Oxford

Info

Catalogue Number 152590
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets Vascular Endothelial Growth Factor (VEGF)
Reactivity Human and Mouse
Relevance Recombinant monoclonal antibody with use investigating MVCD1, angiogenesis and various cancers.

Background and Research Application
VEGF is a 34-43 kD polypeptide growth factor, part of the PDGF family. There are 7 homo-dimeric isoforms (A-G) with VEGF 121, VEGF 165 and VEGF189 being the most extensively studied (F,D and B respectively). They are generated by alternative splicing and binding to VEGF receptors (FLT-1 and KDR) which are selectively expressed on vascular endothelial cells. VEGF elicits mitogenic effects on endothelial cells and is strongly angiogenic, with a role in cancer and metastasis. VEGF is involved in vasculogenesis and endothelial growth. VEGF expression is potentiated, and the protein is secreted by tumour cells in response to hypoxia, by activated oncogenes, growth factors, nitric oxide and a variety of cytokines. Defects in VEGFA are linked to MVCD1 (microvascular complications of diabetes type 1) and VEGF polymorphisms are associated with susceptibility to multiple cancers, e.g. glioma, HCC, ovarian, bladder, prostate, breast cancer etc.
VEGF is an angiogenic growth factor and a prognostic indicator for cancer when detected in serum.
This is a recombinant version of the anti-VEGF monoclonal antibody. This antibody binds VEGF-A.
Host Mouse
Immunogen Human VEGF189 expressed in E. coli.
Subclass IgG1
Formulation PBS only
Concentration 1mg/ml
Positive Control VEGF recombinant protein.
Molecular Weight (kDa) 38-44
Research Area Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Metabolism
Immunogen UniProt ID P15692
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
VG76e will detect both native and denatured protein.
Recombinant monoclonal antibody produced from the original monoclonal. Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma.

Concentration
1mg/ml as standard

References

There are 2 reference entries for this reagent.

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References: 2 entries

Original hybridoma first published in Waterfield et al. 1982. J Cell Biochem. 20(2):149-61. PMID: 6188757.

A monoclonal antibody to the human epidermal growth factor receptor.

Europe PMC ID: 6188757


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References: 2 entries

Original hybridoma first published in Waterfield et al. 1982. J Cell Biochem. 20(2):149-61. PMID: 6188757.

A monoclonal antibody to the human epidermal growth factor receptor.


Add a reference