Anti-CD29 [12G10]
Invented at University of Manchester
- Datasheet
- References (18)
- Inventor Info
Info
![Monolayers of B2bshRNA.luc and B2bshRNA.Sdc1 cells were immunostained for the β1 integrin subunit (red) using all (clone AIIB2) or active conformation-specific (Anti-CD29 [12G10]) antibodies. Immunofluorescent images counterstained with Dapi (scale bar = 100 µm). Taken from Chen et al., 2009. PLoS One. 2009 Aug 10;4(8)](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/53cfdf47-1242-4680-86e5-426e9b7bae1c.png)
![Monolayers of B2bshRNA.luc and B2bshRNA.Sdc1 cells were immunostained for the β1 integrin subunit (red) using all (clone AIIB2) or active conformation-specific (Anti-CD29 [12G10]) antibodies. Immunofluorescent images counterstained with Dapi (scale bar = 100 µm). Taken from Chen et al., 2009. PLoS One. 2009 Aug 10;4(8)](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/53cfdf47-1242-4680-86e5-426e9b7bae1c.png)
![Image thumbnail for Anti-CD29 [12G10]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/53cfdf47-1242-4680-86e5-426e9b7bae1c.png)
| Catalogue Number | 152482 |
| Applications | ELISA FACS IHC IF IP WB |
| Antigen/Gene or Protein Targets | CD29 (Integrin b1 chain) |
| Synonyms | Integrin Subunit Beta 1; Glycoprotein Iia; MSK12; GPIIA; FNRB; MDF2; Very Late Activation Protein; Beta Polypeptide; Fibronectin Receptor Subunit Beta; Integrin VLA-4 Beta Subunit; CD29 Antigen; VLA-BETA; CD29; VLAB |
| Reactivity | Human |
| Relevance | CD29 is a member of the family of integrins, membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. |
| Host | Mouse |
| Immunogen | Purified human β1 integrin preparation from HT1080 fibrosarcoma cell extract. |
| Subclass | IgG1 |
| Myeloma Used | P3X63Ag8.653 |
| Notes |
12G10 has been shown to recognize a ligand-induced binding site of human β1 integrin. The binding is increased in the presence of a fibronectin ligand. This antibody also enhances alpha 5 – beta 1 fibronectin interactions. 12G10 does not cross-react with mouse or rat samples. |
| Research Area | Adhesion, Cancer, Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling, Gene Expression, Immunology |
References: 18 entries
Chen et al. 2009. PLoS One. 4(8):e6565. PMID: 19668337.
IF
MMP7 shedding of syndecan-1 facilitates re-epithelialization by affecting alpha(2)beta(1) integrin activation.
Europe PMC ID: 19668337
Yamamoto et al. 2009. Endocrinology. 150(2):990-9. PMID: 18845630.
N-acetylglucosaminyltransferase V regulates extravillous trophoblast invasion through glycosylation of alpha5beta1 integrin.
Europe PMC ID: 18845630
Yamamoto et al. 2007. Br J Cancer. 97(11):1538-44. PMID: 17971775.
Expression of N-acetylglucosaminyltransferase V in endometrial cancer correlates with poor prognosis.
Europe PMC ID: 17971775
Cavallo-Medved et al. 2005. J Cell Sci. 118(Pt 7):1493-503. PMID: 15769846.
Caveolin-1 mediates the expression and localization of cathepsin B, pro-urokinase plasminogen activator and their cell-surface receptors in human colorectal carcinoma cells.
Europe PMC ID: 15769846
Avizienyte et al. 2002. Nat Cell Biol. 4(8):632-8. PMID: 12134161.
Src-induced de-regulation of E-cadherin in colon cancer cells requires integrin signalling.
Europe PMC ID: 12134161
Iba et al. 2000. J Cell Biol. 149(5):1143-56. PMID: 10831617.
The cysteine-rich domain of human ADAM 12 supports cell adhesion through syndecans and triggers signaling events that lead to beta1 integrin-dependent cell spreading.
Europe PMC ID: 10831617
Mould et al. 1998. Biochem J. 331 ( Pt 3):821-8. PMID: 9560310.
Regulation of integrin function: evidence that bivalent-cation-induced conformational changes lead to the unmasking of ligand-binding sites within integrin alpha5 beta1.
Europe PMC ID: 9560310
Mould et al. 1995. Biochem Soc Trans. 23(3):395S. PMID: 8566283.
Regulation of integrin alpha 5 beta 1 function by anti-integrin antibodies and divalent cations.
Europe PMC ID: 8566283
Mould et al. 1995. FEBS Lett. 363(1-2):118-22. PMID: 7537221.
Identification of a novel anti-integrin monoclonal antibody that recognises a ligand-induced binding site epitope on the beta 1 subunit.
Europe PMC ID: 7537221
Add a reference
References: 18 entries
Chen et al. 2009. PLoS One. 4(8):e6565. PMID: 19668337.
IF
MMP7 shedding of syndecan-1 facilitates re-epithelialization by affecting alpha(2)beta(1) integrin activation.
Yamamoto et al. 2009. Endocrinology. 150(2):990-9. PMID: 18845630.
N-acetylglucosaminyltransferase V regulates extravillous trophoblast invasion through glycosylation of alpha5beta1 integrin.
Yamamoto et al. 2007. Br J Cancer. 97(11):1538-44. PMID: 17971775.
Expression of N-acetylglucosaminyltransferase V in endometrial cancer correlates with poor prognosis.
Cavallo-Medved et al. 2005. J Cell Sci. 118(Pt 7):1493-503. PMID: 15769846.
Caveolin-1 mediates the expression and localization of cathepsin B, pro-urokinase plasminogen activator and their cell-surface receptors in human colorectal carcinoma cells.
Avizienyte et al. 2002. Nat Cell Biol. 4(8):632-8. PMID: 12134161.
Src-induced de-regulation of E-cadherin in colon cancer cells requires integrin signalling.
Iba et al. 2000. J Cell Biol. 149(5):1143-56. PMID: 10831617.
The cysteine-rich domain of human ADAM 12 supports cell adhesion through syndecans and triggers signaling events that lead to beta1 integrin-dependent cell spreading.
Mould et al. 1998. Biochem J. 331 ( Pt 3):821-8. PMID: 9560310.
Regulation of integrin function: evidence that bivalent-cation-induced conformational changes lead to the unmasking of ligand-binding sites within integrin alpha5 beta1.
Mould et al. 1995. Biochem Soc Trans. 23(3):395S. PMID: 8566283.
Regulation of integrin alpha 5 beta 1 function by anti-integrin antibodies and divalent cations.
Mould et al. 1995. FEBS Lett. 363(1-2):118-22. PMID: 7537221.
Identification of a novel anti-integrin monoclonal antibody that recognises a ligand-induced binding site epitope on the beta 1 subunit.
Add a reference
