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HCA-7 Colony27 Cell Line

Info

Parental Line HCA-7
Host Human
Tissue Colon
Disease Keywords Human colorectal adenocarcinoma
Relevance The HCA-7, clolny 27 cell line is a subpopulations isolated from the HCA-7 cell line following butyrate treatment. The HCA-7 cell line is an epithelial human colon adenocarcinoma cell line established from a colon adenocarcinoma of a 58 year-old female patient. The adenocarcinoma was moderately differentiated and determined to be Dukes' stage B.
Production Details Subpopulations isolated from the HCA-7 cell line following butyrate treatment.
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Morphology: Epithelial Growth Mode: Adherent
Recommended Growing Conditions Subculture Routine: Split sub-confluent cultures (70-80%) 1:5 to 1:10 weekly using 0.0125% trypsin/EDTA; 7.5% CO2; 37°C. Suggested seeding density 2-4x10,000 cells/cm².Culture Medium: DMEM (High glucose) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Notes Karyotype: Near diploid
Parent HCA-7 cell line: wildtype APC.
Known mutations:Parent HCA-7 cell line: RER phenotype, p53
Cellosaurus ID CVCL_2473

References: 2 entries

Kirkland et al. 1986. Br J Cancer. 53(6):779-85. PMID: 3718830.

Marsh et al. 1993. J Pathol. 170(4):441-50. PMID: 8410493.


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References: 2 entries

Kirkland et al. 1986. Br J Cancer. 53(6):779-85. PMID: 3718830.

Marsh et al. 1993. J Pathol. 170(4):441-50. PMID: 8410493.


Add a reference

References: 2 entries

Kirkland et al. 1986. Br J Cancer. 53(6):779-85. PMID: 3718830.

Marsh et al. 1993. J Pathol. 170(4):441-50. PMID: 8410493.


Add a reference


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