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HCA-7 Cell Line

Info

Host Human
Tissue Colon
Disease Keywords Human colon adenocarcinoma
Relevance The HCA-7 cell line is an epithelial human colon adenocarcinoma cell line established from a colon adenocarcinoma of a 58 year-old female patient. The adenocarcinoma was moderately differentiated and determined to be Dukes' stage B.
Production Details Established from a colon adenocarcinoma of a 58 year-old female patient. The adenocarcinoma was moderately differentiated and determined to be Dukes' stage B.
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Morphology: EpithelialGrowth Mode: Adherent
Recommended Growing Conditions Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 to 1:10 using 0.25% trypsin/EDTA; 7.5% CO2; 37°C. Suggested seeding density 2-4x10,000 cells/cm².Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Notes DNA Profile: STR-PCR Data:
Amelogenin: X
CSF1PO: 11,14
D13S317: 9
D16S539: 10,11
D5S818: 9,12
D7S820: 11,12
THO1: 6,7
TPOX: 8,10
vWA: 14,16
Wildtype APC.
Known mutations: RER phenotype, p53
Cellosaurus ID CVCL_0289

References: 5 entries

Bicknell et al. 2005. Tissue Antigens. 66(3):231-7. PMID: 16101834.

Ilyas et al. 1997. Proc Natl Acad Sci U S A. 94(19):10330-4. PMID: 9294210.

Kirkland et al. 1986. Br J Cancer. 53(6):779-85. PMID: 3718830.

Liu et al. 2006. Proc Natl Acad Sci U S A. 103(4):976-81. PMID: 16418264.

Wheeler et al. 1999. Proc Natl Acad Sci U S A. 96(18):10296-301. PMID: 10468602.


Add a reference

References: 5 entries

Bicknell et al. 2005. Tissue Antigens. 66(3):231-7. PMID: 16101834.

Ilyas et al. 1997. Proc Natl Acad Sci U S A. 94(19):10330-4. PMID: 9294210.

Kirkland et al. 1986. Br J Cancer. 53(6):779-85. PMID: 3718830.

Liu et al. 2006. Proc Natl Acad Sci U S A. 103(4):976-81. PMID: 16418264.

Wheeler et al. 1999. Proc Natl Acad Sci U S A. 96(18):10296-301. PMID: 10468602.


Add a reference

References: 5 entries

Bicknell et al. 2005. Tissue Antigens. 66(3):231-7. PMID: 16101834.

Ilyas et al. 1997. Proc Natl Acad Sci U S A. 94(19):10330-4. PMID: 9294210.

Kirkland et al. 1986. Br J Cancer. 53(6):779-85. PMID: 3718830.

Liu et al. 2006. Proc Natl Acad Sci U S A. 103(4):976-81. PMID: 16418264.

Wheeler et al. 1999. Proc Natl Acad Sci U S A. 96(18):10296-301. PMID: 10468602.


Add a reference


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