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- Inventor Info
|Antigen/Gene or Protein Targets||p110δ|
|Disease Keywords||Germinal Center Reaction|
Phosphatidylinositol-4,5-Bisphosphate 3-Kinase 110 KDa Catalytic Subunit Delta
p110δ is a member of the PI3K family that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. The PI3K pathway has been implicated in lymphocyte development and activation. p110δ plays a role in oncogenic transformation, and is consistently expressed at a high level in blast cells from AML. Additionally, p110δ signaling pathway is involved in immune responses relevant to the pathogenesis of rheumatoid arthritis and other inflammatory diseases.
As p110δ is expressed by most cells in the immune system, the Cre/LoxP technology is a good tool to identify the cell types that depended on p110δ in the GC reaction.
|Production Details||The pik3cd targeting vector was generated introducing a single LoxP site into the XhoI site upstream of the first coding exon and a neomycin cassette flanked by FRT sites and a single LoxP site (FRTneoFRT) into the EcoRV site between exons 9 and 10. Targeted E14 ES clones were identified by Southern blot analysis of EcoRV digests, which yielded a 5.6-kb fragment for non targeted clones and a 4.2-kb fragment for targeted clones. FLPe was transiently expressed to mediate recombination excision of the neomycin cassette, which was verified by Southern blot analysis. One of the correct clones was selected and injected into blastocysts to produce chimeric mice|
|Conditional Description||Exons 1-9 of p110δ (encoding for the first 490aa of the protein) flanked by LoxP sites for Cre-mediated excision|
|Mouse Genetic Background/Cross History||Backcrossed at least five times to a C57BL/6 background|
|Research Area||Adhesion, Cell Signaling & Signal Transduction, Genetic Studies Tools, Immunology|
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