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Anti-HSV gD [LP2]

Invented by Anthony Minson

Info

Applications FACS IHC IF IP
Antigen/Gene or Protein Targets Herpes Simplex Virus Glycoprotein D (HSV gD)
Reactivity Virus
Relevance The envelope of herpes simplex virus (HSV) is complex, as it contains at least 10 virus-encoded glycoproteins. However, only a subset of these mediate virus entry in cell culture. The initial interaction of HSV with cell surface heparan sulfate proteoglycans is mediated by glycoprotein C (gC) and/or gB. This is presumably followed by interaction of one or more of the viral glycoproteins with cellular receptors. Then gD, gB, and the complex of gH and gL act individually or in combination to trigger pH-independent fusion of the viral envelope with the host cell plasma membrane.
Host Mouse
Immunogen Herpes Simplex Virus Type-1 and Type-2 envelope protein Glycoprotein D
Subclass IgG2a
Notes Clone LP2 strongly neutralises virus in the absence of Complement. Works well for immunofluorescence (IF) on fixed specimens, for immunoprecipitiation (IP) on triton solubilised samples and flow cytometry. Does not work in Western blots. The antibody epitope is well characterised by binding competition assays and by sequencing of antibody escape mutants.
Research Area Virology

References

There are 2 reference entries for this reagent.

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References: 2 entries

Buckmaster et al. 1984. J Med Virol. 13(2):193-202. PMID: 6319591.

Minson et al. 1986. J Gen Virol. 67 ( Pt 6):1001-13. PMID: 2423636.


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References: 2 entries

Buckmaster et al. 1984. J Med Virol. 13(2):193-202. PMID: 6319591.

Minson et al. 1986. J Gen Virol. 67 ( Pt 6):1001-13. PMID: 2423636.


Add a reference

Inventor Information