Anti-Cytochrome P450 4V2 [M29-P3B10]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
- References (4)
- Inventor Info
Info
![Image for Anti-Cytochrome P450 4V2 [M29-P3B10]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/72b4b76d-eafa-4005-b015-53b6615f0809.jpg)
![Image for Anti-Cytochrome P450 4V2 [M29-P3B10]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/e6c9532f-cb00-446d-975d-aa7ac5398728.jpg)
![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded normal and primary colorectal tumour tissue sections using neat culture supernatants of anti-CYP4V2 [M29-P3B10] . Photomicrographs show negative staining of anti-CYP4V2 [M29-P3B10] in normal colon mucosa and strong immunostaining in primary colorectal tumour. Cytoplasmic localisation of CYP4V2 is also observed.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/b0b308bb-5cbc-4f86-942f-a397702ec261.jpg)
![Image for Anti-Cytochrome P450 4V2 [M29-P3B10]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/72b4b76d-eafa-4005-b015-53b6615f0809.jpg)
![Image for Anti-Cytochrome P450 4V2 [M29-P3B10]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/e6c9532f-cb00-446d-975d-aa7ac5398728.jpg)
![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded normal and primary colorectal tumour tissue sections using neat culture supernatants of anti-CYP4V2 [M29-P3B10] . Photomicrographs show negative staining of anti-CYP4V2 [M29-P3B10] in normal colon mucosa and strong immunostaining in primary colorectal tumour. Cytoplasmic localisation of CYP4V2 is also observed.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/b0b308bb-5cbc-4f86-942f-a397702ec261.jpg)
![Image thumbnail for Anti-Cytochrome P450 4V2 [M29-P3B10]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/72b4b76d-eafa-4005-b015-53b6615f0809.jpg)
![Image thumbnail for Anti-Cytochrome P450 4V2 [M29-P3B10]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/e6c9532f-cb00-446d-975d-aa7ac5398728.jpg)
![Image thumbnail for Anti-Cytochrome P450 4V2 [M29-P3B10]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/b0b308bb-5cbc-4f86-942f-a397702ec261.jpg)
| Catalogue Number | 152159 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Cytochrome P450, family 4, subfamily V, polypeptide 2 (CYP4V2) |
| Synonyms | Cytochrome P450; Family 4; Subfamily V; Polypeptide 2; Docosahexaenoic Acid Omega-Hydroxylase CYP4V2; CYP4AH1; BCD |
| Reactivity | Human |
| Relevance | Cytochrome P450, family 4, subfamily V, polypeptide 2 (CYP4V2) encodes a member of the cytochrome P450 superfamily of enzymes that are a group of heme-thiolate monooxygenases. Cytochromes P450 catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP4V2 catalyzes the omega-hydroxylation of medium-chain saturated fatty acids such as laurate, myristate and palmitate in an NADPH-dependent pathway. CYP4V2 may have a role in fatty acid and steroid metabolism in the eye and its mutations are associated with Bietti's crystalline dystrophy (BCD) and retinitis pigmentosas. |
| Host | Mouse |
| Immunogen | Peptide sequence – KREELGLEGQ (amino acids 495 – 504) |
| Positive Control | ELISA- Peptide immunogen; Western Blot- Human liver microsomes; IHC- Human liver |
| Subclass | IgG1 kappa |
| Myeloma Used | P3X63Ag8.653 |
| Recommended Growing Conditions | Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine + 3ml of Penicillin -Streptomycin. |
| Strain | Balb/c |
| Research Area | Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Metabolism |
References: 4 entries
Alnabulsi et al. 2017. Br J Cancer. :. PMID: 28557975.
The differential expression of omega-3 and omega-6 fatty acid metabolising enzymes in colorectal cancer and its prognostic significance.
Europe PMC ID: 28557975
Alnabulsi et al. 2016. Characterisation of Arachidonic Acid Metabolising Enzymes in Colorectal Cancer. J Pathol. 240 Suppl 1:S1-S48. PMID: 27747872
IHC
Nottingham Pathology 2016. 9th Joint Meeting of the British Division of the International Academy of Pathology and the Pathological Society of Great Britain & Ireland, 28 June - 1 July 2016.
Europe PMC ID: 27747872
Add a reference
References: 4 entries
Alnabulsi et al. 2017. Br J Cancer. :. PMID: 28557975.
The differential expression of omega-3 and omega-6 fatty acid metabolising enzymes in colorectal cancer and its prognostic significance.
Alnabulsi et al. 2016. Characterisation of Arachidonic Acid Metabolising Enzymes in Colorectal Cancer. J Pathol. 240 Suppl 1:S1-S48. PMID: 27747872
IHC
Nottingham Pathology 2016. 9th Joint Meeting of the British Division of the International Academy of Pathology and the Pathological Society of Great Britain & Ireland, 28 June - 1 July 2016.
Add a reference
