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Anti-HHV-8 LNA-1 [LN53]

Info

Catalogue Number 152152
Applications ELISA FACS IHC IP WB
Antigen/Gene or Protein Targets Latent nuclear antigen 1 (LNA-1; ORF73) of HHV-8
Synonyms ORF-73 of human herpesvirus 8 or HHV-8 positive cells
Reactivity Virus
Relevance Rat monoclonal antibody which targets human KSHV/HHV8 and simian RFHV Orf73 latency-associated nuclear antigen (LANA or LNA) with high specificity.

Background and Research Application
Human herpesvirus-8 (HHV-8, also called KSHV) has been found to be associated with three different diseases observed in AIDS patients; Kaposi's sarcoma (KS), primary effusion lymphoma ( PEL, a rare type of non-Hodgkin lymphoma affecting the body cavities) and multicentric Castleman's disease (MCD). To date there is much evidence to support a direct role for HHV-8 in KS. In early KS, HHV-8 is present in 90% of spindle cells, but not in normal vascular endothelium. HHV-8 encodes a latent nuclear antigen (LNA-1) which is the product of the viral gene Orf73. This is the target of anti-HHV-8 LNA-1.

Anti-HHV-8 LNA-1 recognizes an EQEQE repeat epitope in Orf73 and reacts with antigens in paraffin-embedded tissue.
LNA-1 is a nuclear protein which functions to ensure the maintenance of the viral genome by tethering the viral episomal DNA to host cell chromosomes. LNA-1 also regulates the cellular transcription program via interaction with a number of cellular proteins. These include transcriptional regulators and known tumour suppressors, p53 and retinoblastoma (RB). This nuclear protein is also involved in maintaining the latent state of the virus by inhibiting viral replication.

LNA-1 is recognized by most infected patient sera and is the basis of current immunofluorescence assays used in epidemiological studies of HHV-8 infection. Orf73 encodes the major immunogenic LNA-1, identified as a nuclear stippling pattern in cells when exposed to sera of patients with KS.
Host Rat
Immunogen Recombinant protein corresponding to the latent nuclear antigen-1 molecule of HHV-8
Immunogen UniProt ID Q9QR71
Subclass IgG2c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
4 monoclonal antibodies were produced in the original development - LN53 was the most reactive and robust for LNA-1 antigen in sections from paraffin-embedded biopsies and works well for immunohistochemistry.

LN53 recognizes the epitope EQEQE. The LN53 epitope is contained within the repetitive region of LNA-1, resulting in 23 copies of the LN53 epitope in full-length LNA-1.

LN53 reacts to Human herpesvirus 8.

Concentration
1mg/ml as standard
Research Area Virology

References

There are 10 reference entries for this reagent.

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References: 10 entries

Juillard et al. 2019. J Virol. 93(6):. PMID: 30626680.

Pires de Miranda et al. 2018. J Virol. 92(21):. PMID: 30111565.

Grant et al. 2018. Proc Natl Acad Sci U S A. 115(19):4992-4997. PMID: 29610353.

Garrigues et al. 2017. J Virol. 91(24):. PMID: 28978712.

Bruce et al. 2017. Pathogens. 6(1):. PMID: 28335496.

Brouchet et al. 2005. Br J Cancer. 92(4):743-6. PMID: 15700034.

IHC

Caussinus et al. 2001. J Clin Pathol. 54(10):790-1. PMID: 11577129.

IHC

Gomez-Brouchet et al. 2001. J Clin Pathol. 54(8):617-8. PMID: 11477117.

IHC

Kellam et al. 1999. J Virol. 73(6):5149-55. PMID: 10233979.

ELISA WB FACS IHC IP

Dupin et al. 1999. Proc Natl Acad Sci U S A. 96(8):4546-51. PMID: 10200299.

IHC


Add a reference

References: 10 entries

Juillard et al. 2019. J Virol. 93(6):. PMID: 30626680.

Pires de Miranda et al. 2018. J Virol. 92(21):. PMID: 30111565.

Grant et al. 2018. Proc Natl Acad Sci U S A. 115(19):4992-4997. PMID: 29610353.

Garrigues et al. 2017. J Virol. 91(24):. PMID: 28978712.

Bruce et al. 2017. Pathogens. 6(1):. PMID: 28335496.

Brouchet et al. 2005. Br J Cancer. 92(4):743-6. PMID: 15700034.

IHC

Caussinus et al. 2001. J Clin Pathol. 54(10):790-1. PMID: 11577129.

IHC

Gomez-Brouchet et al. 2001. J Clin Pathol. 54(8):617-8. PMID: 11477117.

IHC

Kellam et al. 1999. J Virol. 73(6):5149-55. PMID: 10233979.

ELISA WB FACS IHC IP

Dupin et al. 1999. Proc Natl Acad Sci U S A. 96(8):4546-51. PMID: 10200299.

IHC


Add a reference

Inventor Information

No inventors are currently linked to this reagent.

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