Human CIHGM-1 Cell Line
Invented by Prof Moin Saleem from University Of Bristol
Invented at University Of Bristol
- Datasheet
- References (2)
- Inventor Info
Info
| Antigen/Gene or Protein Targets | Conditionally immortalised Glomerular Mesangial Cell Line |
| Host | Human |
| Tissue | Kidney |
| Model | Primary line |
| Relevance | The Human CIHGM-1 Cell Line is a primary line isolated from human kidneys. Mesangial cells form the central structural component of the glomerulus, with mesangial cell injury a characteristic of numerous renal diseases. Consequently, mesangial cells are a key therapeutic target in the treatment of human glomerular disease. Extensively characterised and published, these cells represent the most physiologically relevant model in which to study human glomerular disease in-vitro, with the conditionally immortalised nature of the cell line enabling an in-vitro process of differentiation equivalent to the in-vivo differentiation of mesangial cells. Resulting cells express key mesangial markers including fibronectin, -smooth muscle actin and PDGF -R. |
| Conditional Description | Immortalization is conditional by use of a temperature-sensitive transgene encoding SV40 |
| Research Area | Other |
| Recommended Growing Conditions | RPMI 1640 medium supplemented with 10% fetal calf serum, 10 U/ml streptomycin, 10 U/ml penicillin, and ITS |
| Notes |
Applications: Toxicology studies Metabolism Therapeutic targeting Renal physiology R&D Co-culture studies (e.g 3D culture model) Key benefits: Primary human cell derived Physiologically relevant phenotype analogous to in-vivo differentiated renal cells Homogenous, stable and long lasting |
| Cellosaurus ID | CVCL_W168 |
References: 2 entries
Sarrab et al. 2011. Am J Physiol Renal Physiol. 301(5):F1131-8. PMID: 21653636.
Establishment of conditionally immortalized human glomerular mesangial cells in culture, with unique migratory properties.
Europe PMC ID: 21653636
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References: 2 entries
Sarrab et al. 2011. Am J Physiol Renal Physiol. 301(5):F1131-8. PMID: 21653636.
Establishment of conditionally immortalized human glomerular mesangial cells in culture, with unique migratory properties.
Add a reference
