Human CiGEnC Cell Line
Invented at University Of Bristol
- Datasheet
- References (2)
- Inventor Info
Info
| Antigen/Gene or Protein Targets | Conditionally immortalised Glomerular Endothelial Cell Line |
| Host | Human |
| Tissue | Kidney |
| Model | Primary line |
| Relevance | The Human CiGEnC Line is a conditionally immortalised GEnC line that allows the in-vitro differentiation of cells in a similar manner to in-vivo, without the onset of early senescence which limits other GEnC lines. This novel, stable, easy to culture cell line has key characteristics of GEnCs, including expression of markers such as PECAM1, ICAM2, VEGFR2, vWF, and, uniquely for a cell line, upregulates fenestrations in response to VEGF. GEnCs are considered to be a gold standard in glomerular filtration research. GEnCs are highly specialised cells which form the inner layer of glomerular capillaries as part of the glomerular microcirculation. They are an essential component in glomerular filtration. With damage of GEnCs implicated in diabetic nephropathy, haemolytic uraemic syndrome and preeclampsia, in-vitro study of GEnCs is vital to gain understanding of such diseases. However, as a difficult primary cell to culture, study to date has been limited. |
| Conditional | Yes |
| Conditional Description | Immortalization is conditional by use of a temperature-sensitive transgene encoding SV40LT |
| Research Area | Other |
| Notes |
Applications: Toxicology studies Metabolism Therapeutic targeting Renal physiology R&D Co-culture studies (e.g 3D culture model) Key benefits: Primary human cell derived Physiologically relevant phenotype analogous to in-vivo differentiated renal cells Homogenous, stable and long lasting |
| Cellosaurus ID | CVCL_W185 |
References: 2 entries
Satchell et al. 2006. Kidney Int. 69(9):1633-40. PMID: 16557232.
Conditionally immortalized human glomerular endothelial cells expressing fenestrations in response to VEGF.
Europe PMC ID: 16557232
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References: 2 entries
Satchell et al. 2006. Kidney Int. 69(9):1633-40. PMID: 16557232.
Conditionally immortalized human glomerular endothelial cells expressing fenestrations in response to VEGF.
Add a reference
