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Murine Podocyte Cell Line

Invented by Prof Moin Saleem at University Of Bristol

Info

Host Mouse
Tissue Kidney
Model Primary line
Relevance The conditionally immortalised murine podocyte cell line allows an in-vitro process of maturation analogous to the development and maturation of podocytes in-vivo. The result is a homogenous, stable cell source that shows expression of key antigenic markers of differentiated in-vivo podocytes. These include the novel podocyte proteins nephrin, podocin, CD2AP and synaptopodin.
Conditional Description Immortalization is conditional by use of a temperature-sensitive transgene encoding SV40LT
Research Area Other
Recommended Growing Conditions RPMI-1640, Insulin-Transferrin-Selenium, Foetal Bovine Serum (10% v/v), Pen/Strep medium at at 33°C or 37°C, 5% CO2.
Notes Applications:
Toxicology studies
Metabolism
Therapeutic targeting
Renal physiology R&D
Co-culture studies (e.g 3D culture model)

Key benefits:
Physiologically relevant phenotype analogous to in-vivo differentiated renal cells
Homogenous, stable and long lasting
Cellosaurus ID CVCL_AS87

References: 2 entries

Ni et al. 2012. Nephrology (Carlton). 17(6):525-31. PMID: 22591222.

Mundel et al. 1997. Exp Cell Res. 236(1):248-58. PMID: 9344605.


Add a reference

References: 2 entries

Ni et al. 2012. Nephrology (Carlton). 17(6):525-31. PMID: 22591222.

Mundel et al. 1997. Exp Cell Res. 236(1):248-58. PMID: 9344605.


Add a reference

References: 2 entries

Ni et al. 2012. Nephrology (Carlton). 17(6):525-31. PMID: 22591222.

Mundel et al. 1997. Exp Cell Res. 236(1):248-58. PMID: 9344605.


Add a reference


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