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Murine Podocyte Cell Line

Invented by Prof Moin Saleem at University Of Bristol

Info

Host Mouse
Tissue Kidney
Model Primary line
Relevance Cell line to study the determinative steps of podocyte differentiation and process formation.

Background and Research Application
The conditionally immortalised murine podocyte cell line allows an in-vitro process of maturation analogous to the development and maturation of podocytes in-vivo. Both proliferating and differentiating MPC cells express the WT-1 protein and an ordered array of actin fibres and microtubules extends into the forming cellular processes during differentiation, reminiscent of podocyte processes in vivo. The result is a homogenous, stable cell source that shows expression of key antigenic markers of differentiated in-vivo podocytes. These include the novel podocyte proteins nephrin, podocin, CD2AP and synaptopodin.
Conditional Description Immortalization is conditional by use of a temperature-sensitive transgene encoding SV40LT
Research Area Other
Recommended Growing Conditions RPMI-1640, Insulin-Transferrin-Selenium, Foetal Bovine Serum (10% v/v), Pen/Strep medium at at 33°C or 37°C, 5% CO2.
Notes Points of Interest
Applications:
Toxicology studies
Metabolism
Therapeutic targeting
Renal physiology R&D
Co-culture studies (e.g 3D culture model)

Key benefits:
Physiologically relevant phenotype analogous to in-vivo differentiated renal cells
Homogenous, stable and long lasting

Concentration
Vial has between 1-5 million cells as standard, however this may vary.
Cellosaurus ID CVCL_AS87

References

There are 9 reference entries for this reagent.

View All References

References: 9 entries

Peng et al. 2019. Exp Ther Med. 18(6):4829-4837. PMID: 31798708.

Che et al. 2019. PeerJ. 7:e8016. PMID: 31799068.

Khullar et al. 2018. Clin Exp Nephrol. 22(3):508-516. PMID: 29022109.

Bettaieb et al. 2017. Biochim Biophys Acta Gen Subj. 1861(11 Pt A):2758-2765. PMID: 28757338.

Zea et al. 2016. PLoS One. 11(7):e0157907. PMID: 27389192.

Ni et al. 2012. Nephrology (Carlton). 17(6):525-31. PMID: 22591222.

Podocyte culture: tricks of the trade.

Europe PMC ID: 22591222

Mundel et al. 1997. Exp Cell Res. 236(1):248-58. PMID: 9344605.

Rearrangements of the cytoskeleton and cell contacts induce process formation during differentiation of conditionally immortalized mouse podocyte cell lines.

Europe PMC ID: 9344605


Add a reference

References: 9 entries

Peng et al. 2019. Exp Ther Med. 18(6):4829-4837. PMID: 31798708.

Che et al. 2019. PeerJ. 7:e8016. PMID: 31799068.

Khullar et al. 2018. Clin Exp Nephrol. 22(3):508-516. PMID: 29022109.

Bettaieb et al. 2017. Biochim Biophys Acta Gen Subj. 1861(11 Pt A):2758-2765. PMID: 28757338.

Zea et al. 2016. PLoS One. 11(7):e0157907. PMID: 27389192.

Ni et al. 2012. Nephrology (Carlton). 17(6):525-31. PMID: 22591222.

Podocyte culture: tricks of the trade.

Mundel et al. 1997. Exp Cell Res. 236(1):248-58. PMID: 9344605.

Rearrangements of the cytoskeleton and cell contacts induce process formation during differentiation of conditionally immortalized mouse podocyte cell lines.


Add a reference