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Monoclonal Antibodies

Anti-Cytochrome P450 2R1 [M26-P6H1]

Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
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Western Blot using mAb M26P6H1
Immnohistochemistry: wax-embedded human kidney
Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections and showed positive cytoplasmic immunostaining in normal liver (A) and normal kidney (B) as reported by literature. Primary colorectal (C) and metastatic (D) colorectal tumors showed moderate staining compared whilst normal colon mucosa was negatively stained.
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Catalogue Number 152121
Applications ELISA IHC WB
Antigen/Gene or Protein Targets CYP2R1, Cytochrome P450, family 2, subfamily R, polypeptide 1.
Reactivity Human and Mouse
Relevance Cytochrome P450 2R1 (CYP2R1) encodes a member of the cytochrome P450 superfamily of enzymes that are a group of heme-thiolate monooxygenases. Cytochromes P450 catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP2R1 expressed in the liver is a microsomal vitamin D hydroxylase that converts vitamin D into 25-hydroxyvitamin D (calcidiol), the major circulatory form of the vitamin.
Host Mouse
Immunogen Ovalbumin-conjugated synthetic peptide QPYLICAERR (C-terminal sequence).
Positive Control IHC: formalin-fixed, paraffin-embedded human kidney sections. WB: pooled liver microsomes.
Subclass IgG1 lambda
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).
Notes This antibody was applied in a poster presented at the 2015 NCRI conference: http://abstracts.ncri.org.uk/?s=oxysterol&year_published=2015
Research Area Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Metabolism

References

There are 2 reference entries for this reagent.

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References: 2 entries

Characterisation of the oxysterol metabolising enzyme pathway in mismatch repair proficient and deficient colorectal cancer.

Europe PMC ID: 27341022

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Swan et al. 2016. Oncotarget. :. PMID: 27341022.

IHC WB

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References: 2 entries

Characterisation of the oxysterol metabolising enzyme pathway in mismatch repair proficient and deficient colorectal cancer.

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Swan et al. 2016. Oncotarget. :. PMID: 27341022.

IHC WB

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Inventor Information