Anti-Cytochrome P450 26B1 [T5P3G2*A3]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
- References (2)
- Inventor Info
Info
![Image for Anti-Cytochrome P450 26B1 [T5P3G2*A3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/e6c21f5e-c8c2-4d50-b61a-805678af7f48.jpg)
![Image for Anti-Cytochrome P450 26B1 [T5P3G2*A3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/3752e74e-3e34-4e64-93a9-26fc09249c48.jpg)
![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections. The image is showing a strong cytoplamic positivity of CYP26B1 in primary colorectal cancer using Anti-CYP26B1 [T5P3G2*A3]. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/69ff6462-cdb6-4250-8bab-46920a8c0cf5.jpg)
![Image for Anti-Cytochrome P450 26B1 [T5P3G2*A3]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/e6c21f5e-c8c2-4d50-b61a-805678af7f48.jpg)
![Image for Anti-Cytochrome P450 26B1 [T5P3G2*A3]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/3752e74e-3e34-4e64-93a9-26fc09249c48.jpg)
![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections. The image is showing a strong cytoplamic positivity of CYP26B1 in primary colorectal cancer using Anti-CYP26B1 [T5P3G2*A3]. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/69ff6462-cdb6-4250-8bab-46920a8c0cf5.jpg)
![Image thumbnail for Anti-Cytochrome P450 26B1 [T5P3G2*A3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/e6c21f5e-c8c2-4d50-b61a-805678af7f48.jpg)
![Image thumbnail for Anti-Cytochrome P450 26B1 [T5P3G2*A3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/3752e74e-3e34-4e64-93a9-26fc09249c48.jpg)
![Image thumbnail for Anti-Cytochrome P450 26B1 [T5P3G2*A3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/69ff6462-cdb6-4250-8bab-46920a8c0cf5.jpg)
| Catalogue Number | 152120 |
| Applications | IHC WB |
| Antigen/Gene or Protein Targets | Cytochrome P450, family 2, subfamily A, polypeptide 6, CYP26B1 |
| Reactivity | Human, Mouse and Rat |
| Relevance | Involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation. Involved in the specific inactivation of all-trans-retinoic acid (all-trans-RA), with a preference for the following substrates: all-trans-RA > 9-cis-RA > 13-cis-RA. Generates several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA, and 18-OH-RA. Esential for postnatal survival. Plays a central role in germ cell development: acts by degrading RA in the developing testis, preventing STRA8 expression, thereby leading to delay of meiosis. Required for the maintenance of the undifferentiated state of male germ cells during embryonic development in Sertoli cells, inducing arrest in G0 phase of the cell cycle and preventing meiotic entry. Plays a role in skeletal development, both at the level of patterning and in the ossification of bone and the establishment of some synovial joints. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptide DSNQNEILPE (C-terminal sequence) |
| Positive Control | IHC: formalin-fixed, paraffin-embedded colon carcinoma sections. western blot: overexpression lysates. |
| Subclass | IgG1 kappa |
| Myeloma Used | P3X63Ag8.653 |
| Recommended Growing Conditions | Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine). |
| Strain | Balb/c |
| Research Area | Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Metabolism |
References: 2 entries
Brown et al. 2014. PLoS One. 9(3):e90776. PMID: 24608339.
The expression and prognostic significance of retinoic acid metabolising enzymes in colorectal cancer.
Europe PMC ID: 24608339
Add a reference
References: 2 entries
Brown et al. 2014. PLoS One. 9(3):e90776. PMID: 24608339.
The expression and prognostic significance of retinoic acid metabolising enzymes in colorectal cancer.
Add a reference
