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Anti-Cytochrome P450 26B1 [T5P3G2*A3]

Invented by Ayham Alnabulsi at Vertebrate Antibodies Limited

Info

Catalogue Number 152120
Applications IHC WB
Antigen/Gene or Protein Targets Cytochrome P450, family 2, subfamily A, polypeptide 6, CYP26B1
Reactivity Human, Mouse and Rat
Relevance Involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation. Involved in the specific inactivation of all-trans-retinoic acid (all-trans-RA), with a preference for the following substrates: all-trans-RA > 9-cis-RA > 13-cis-RA. Generates several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA, and 18-OH-RA. Esential for postnatal survival. Plays a central role in germ cell development: acts by degrading RA in the developing testis, preventing STRA8 expression, thereby leading to delay of meiosis. Required for the maintenance of the undifferentiated state of male germ cells during embryonic development in Sertoli cells, inducing arrest in G0 phase of the cell cycle and preventing meiotic entry. Plays a role in skeletal development, both at the level of patterning and in the ossification of bone and the establishment of some synovial joints.
Host Mouse
Immunogen Ovalbumin-conjugated synthetic peptide DSNQNEILPE (C-terminal sequence)
Subclass IgG1 kappa
Positive Control IHC: formalin-fixed, paraffin-embedded colon carcinoma sections. western blot: overexpression lysates.
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).
Strain Balb/c
Research Area Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Metabolism

References: 1 entry

Brown et al. 2014. PLoS One. 9(3):e90776. PMID: 24608339.


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References: 1 entry

Brown et al. 2014. PLoS One. 9(3):e90776. PMID: 24608339.


Add a reference

References: 1 entry

Brown et al. 2014. PLoS One. 9(3):e90776. PMID: 24608339.


Add a reference


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