Anti-Cytochrome P450 7B1 [M17-P3F2]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
- References (2)
- Inventor Info
Info
![Western blot was performed using anti-Cytochrome P450 7B1 [M17-P3F2]. The left hand lane contains control cell lysate while the right hand lane contains lysate prepared from cells over-expressing the protein. ~15 micrograms of protein were loaded per well. No bands were detected with the negative control lysate, while a band migrating at the expected molecular weight was observed in the over-expression lysate.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/ab1fc0d3-6398-4ad4-a17f-9336f8d67e8c.png)
![Western blot was performed using anti-Cytochrome P450 7B1 [M17-P3F2]. The left hand lane contains control cell lysate while the right hand lane contains lysate prepared from cells over-expressing the protein. ~15 micrograms of protein were loaded per well. No bands were detected with the negative control lysate, while a band migrating at the expected molecular weight was observed in the over-expression lysate.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/ab1fc0d3-6398-4ad4-a17f-9336f8d67e8c.png)
![Image thumbnail for Anti-Cytochrome P450 7B1 [M17-P3F2]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/6877fc3b-6d50-41de-abb0-f3ad76ede323.jpg)
![Image thumbnail for Anti-Cytochrome P450 7B1 [M17-P3F2]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/ab1fc0d3-6398-4ad4-a17f-9336f8d67e8c.png)
| Catalogue Number | 152117 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1) |
| Reactivity | Human |
| Relevance |
Defects in CYP7B1 are the cause of spastic paraplegia autosomal recessive type 5A (SPG5A) [MIM:270800]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Defects in CYP7B1 are the cause of congenital bile acid synthesis defect type 3 (CBAS3) [MIM:613812]. Clinical features include severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptide IQYPDSDVL (C-terminal sequence) |
| Positive Control | IHC: formalin-fixed, paraffin-embedded human liver sections WB: pooled human liver microsomes |
| Subclass | IgG1 lambda |
| Myeloma Used | P3X63Ag8.653 |
| Strain | Balb/c |
| Notes | This antibody was applied in a poster presented at the 2015 NCRI conference: http://abstracts.ncri.org.uk/?s=oxysterol&year_published=2015 |
| Research Area | Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Metabolism, Neurobiology |
References: 2 entries
Characterisation of the oxysterol metabolising enzyme pathway in mismatch repair proficient and deficient colorectal cancer.
Europe PMC ID: 27341022
Swan et al. 2016. Oncotarget. :. PMID: 27341022.
IHC WB
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References: 2 entries
Characterisation of the oxysterol metabolising enzyme pathway in mismatch repair proficient and deficient colorectal cancer.
Swan et al. 2016. Oncotarget. :. PMID: 27341022.
IHC WB
Add a reference
