Anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9]
Invented by Alfred Nijkerk from UbiQ Bio
Invented at UbiQ Bio
- Datasheet
- References (4)
- Inventor Info
Info
![Image for Anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/968ab48a-9088-41a3-91a2-b37bbef10764.jpg)
![Adapted from Vlaming et al. 2016. Elife. 5:. PMID: 27922451.: ChIP using anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9].
Gcn5 regulates H2Bub and H3K9ac at several loci. ChIP-qPCR analysis of H2Bub and H3K9ac, normalized to H2B, at several loci. n=3 each, the wild-type average was set to 1.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/81f589d2-68c0-4b1c-bbf1-e3e321b8fee9.jpg)
![Image for Anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/968ab48a-9088-41a3-91a2-b37bbef10764.jpg)
![Adapted from Vlaming et al. 2016. Elife. 5:. PMID: 27922451.: ChIP using anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9].
Gcn5 regulates H2Bub and H3K9ac at several loci. ChIP-qPCR analysis of H2Bub and H3K9ac, normalized to H2B, at several loci. n=3 each, the wild-type average was set to 1.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/81f589d2-68c0-4b1c-bbf1-e3e321b8fee9.jpg)
![Image thumbnail for Anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/968ab48a-9088-41a3-91a2-b37bbef10764.jpg)
![Image thumbnail for Anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/dc7fac18-1b9c-48e4-803f-dcab2d51a142.png)
![Image thumbnail for Anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/dfcffd6b-f69c-438a-a329-227cb29064db.png)
![Image thumbnail for Anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/bef9e519-93f5-4b7d-8101-4fcca380fc01.jpg)
![Image thumbnail for Anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/81f589d2-68c0-4b1c-bbf1-e3e321b8fee9.jpg)
| Catalogue Number | 152107 |
| Applications | ChIP ELISA WB |
| Antigen/Gene or Protein Targets | Yeast monoubiquityl-histone H2B |
| Reactivity | Human, Schizosaccharomyces pombe and Saccharomyces cerevisiae |
| Relevance | C-terminus monoubiquitination of histone H2B acts as a signal for downstream processes such as gene regulation, genome packaging, histone modification crosstalk, and DNA repair. How the ubiquitination levels are controlled and how the Ub signal is translated to downstream effects is poorly understood. An antibody that detects K123 monoubiquitinated yeast H2B, homologue to human H2B K120, will enable research to discover the fundamental principles of the regulation and function of monoubiquitination of H2B. The antibody is developed using an ubiquitin-polypeptide antigen based on the amino-acids 115-130 of the yeast H2B protein, linked by a proteolytically stable bond. |
| Host | Mouse |
| Immunogen | Ub-polypeptide antigen based on amino acids 115 - 130 of yeast H2B |
| Positive Control | Yeast histone preparation |
| Subclass | IgG1 |
| Molecular Weight (kDa) | 23 |
| Notes | Clone formerly called "H2BK123" |
| Research Area | DNA Damage and Repair, Epigenetics & Nuclear Signalling |
References: 4 entries
van Welsem et al. 2018. Nucleic Acids Res. 46(21):11251-11261. PMID: 30203048.
WB
Dot1 promotes H2B ubiquitination by a methyltransferase-independent mechanism.
Europe PMC ID: 30203048
Vlaming et al. 2016. Elife. 5:. PMID: 27922451.
ChIP WB
Direct screening for chromatin status on DNA barcodes in yeast delineates the regulome of H3K79 methylation by Dot1.
Europe PMC ID: 27922451
Add a reference
References: 4 entries
van Welsem et al. 2018. Nucleic Acids Res. 46(21):11251-11261. PMID: 30203048.
WB
Dot1 promotes H2B ubiquitination by a methyltransferase-independent mechanism.
Vlaming et al. 2016. Elife. 5:. PMID: 27922451.
ChIP WB
Direct screening for chromatin status on DNA barcodes in yeast delineates the regulome of H3K79 methylation by Dot1.
Add a reference
