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pIRES-mRFP-NLS-PCNA (2514) Vector

Invented by Dr Jonathon Pines at Gurdon Institute

Info

Antigen/Gene or Protein Targets PCNA
Relevance Species: H. sapiens (human)
Promoter: CMV
Tags / Fusion Proteins:
• NLS (N terminal on backbone)
• mRFP (N terminal on backbone)

Cloning Information
Cloning method: Unknown
5′ cloning site: NdeI (not destroyed)
3′ cloning site: NotI (not destroyed)
5′ sequencing primer: CMF-F
Research Area DNA Damage and Repair, Epigenetics & Nuclear Signalling
Notes Backbone
Vector backbone: pIRESneo
Backbone manufacturer: Clontech
Vector type: Mammalian Expression
Selectable markers: Neomycin

Growth in Bacteria
Bacterial Resistance(s): Ampicillin
Growth Temperature: 37°C
Growth Strain(s): DH5alpha
Copy number: Unknown

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