Anti-BCLAF1 [M33-P5B11]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
- References (2)
- Inventor Info
Info
![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections using Anti-BCLAF1 [M33-P5B11]. Image is showing both cytoplasmic and nuclear localisation positivity of BCLAF1 in colon cancer which is in agreement with existing literature. Exclusively nuclear localisation of BCLAF1 is observed in non-neoplastic colon mucosa. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/7bad435b-1910-436e-916c-93f2175ea0b5.jpg)
![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections using Anti-BCLAF1 [M33-P5B11]. Image is showing both cytoplasmic and nuclear localisation positivity of BCLAF1 in colon cancer which is in agreement with existing literature. Exclusively nuclear localisation of BCLAF1 is observed in non-neoplastic colon mucosa. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/7bad435b-1910-436e-916c-93f2175ea0b5.jpg)
![Image thumbnail for Anti-BCLAF1 [M33-P5B11]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/842b1d70-8300-4a8f-8c9b-b33681b31d0f.jpg)
![Image thumbnail for Anti-BCLAF1 [M33-P5B11]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/e3a8ca32-b3e9-4153-8b21-511b5efba7d1.png)
![Image thumbnail for Anti-BCLAF1 [M33-P5B11]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/7bad435b-1910-436e-916c-93f2175ea0b5.jpg)
| Catalogue Number | 151874 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | BCL2 Associated Transcription Factor 1 (BCLAF1) |
| Reactivity | Human |
| Relevance | BCLAF1 was associated with poor prognosis and showed aberrant localization in colorectal cancer. |
| Host | Mouse |
| Immunogen | Peptide Sequence - KYQGDGIVED (amino acids 900 – 909) |
| Positive Control | ELISA- Peptide immunogenWestern Blot- Hela whole cell extractIHC- Human colon carcinoma |
| Subclass | IgG1 kappa |
| Recommended Growing Conditions | Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine(Sigma G7513) + 3ml of Penicillin -Streptomycin (Sigma P0781). |
| Strain | Balb/c |
| Research Area | Apoptosis and Programmed Cell Death, Cancer, Epigenetics & Nuclear Signalling |
References: 2 entries
Brown et al. 2016. Histopathology. 68(4):556-66. PMID: 26183150.
IHC WB
The expression and prognostic significance of bcl-2-associated transcription factor 1 in rectal cancer following neoadjuvant therapy.
Europe PMC ID: 26183150
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References: 2 entries
Brown et al. 2016. Histopathology. 68(4):556-66. PMID: 26183150.
IHC WB
The expression and prognostic significance of bcl-2-associated transcription factor 1 in rectal cancer following neoadjuvant therapy.
Add a reference
