Anti-SPRED1 [M23-P2G3]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
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Info
Catalogue Number | 151873 |
Applications | ELISA IF WB |
Antigen/Gene or Protein Targets | Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) |
Reactivity | Human |
Relevance | Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. SPRED1 can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in SPRED1 can cause neurofibromatosis type 1-like syndrome (NFLS) and contribute to Legius syndrome and childhood leukemia. |
Host | Mouse |
Immunogen | Peptide Sequence YVPLRMCHR (amino acids 420 – 428) |
Positive Control | ELISA- Peptide immunogenWestern Blot- Hela whole cell extractImmunofluorescence- Hela Cells |
Subclass | IgG1 kappa |
Myeloma Used | P3X63Ag8.653 |
Recommended Growing Conditions | Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine + 3ml of Penicillin -Streptomycin. |
Strain | Balb/c |
Research Area | Cancer, Cell Signaling & Signal Transduction |
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