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Anti-SPRED1 [M23-P2G3]

Invented by Ayham Alnabulsi at Vertebrate Antibodies Limited

Info

Catalogue Number 151873
Applications ELISA IF WB
Antigen/Gene or Protein Targets Sprouty-Related, EVH1 Domain Containing 1 (SPRED1)
Reactivity Human
Relevance Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. SPRED1 can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in SPRED1 can cause neurofibromatosis type 1-like syndrome (NFLS) and contribute to Legius syndrome and childhood leukemia.
Host Mouse
Immunogen Peptide Sequence YVPLRMCHR (amino acids 420 – 428)
Subclass IgG1 kappa
Positive Control ELISA- Peptide immunogenWestern Blot- Hela whole cell extractImmunofluorescence- Hela Cells
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine + 3ml of Penicillin -Streptomycin.
Strain Balb/c
Research Area Cancer, Cell Signaling & Signal Transduction

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