Anti-DRAM1 [M3-P4B4]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
- References (2)
- Inventor Info
Info
![Image for Anti-DRAM1 [M3-P4B4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/1f0a774e-bf8f-4328-b635-45aca1d303ae.jpg)
![Image for Anti-DRAM1 [M3-P4B4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/601fd023-6e43-42b2-8a8a-fae52d472ecc.jpg)
![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue normal colon mucosa, primary tumour and lymph node metastasis sections using anti-DRAM-1 [M3-P4B4].](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/45c76ff1-e785-41e4-b8ac-37b59edbb6e5.jpg)
![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections. Positive cytoplasmic staining is observed in hepatocellualr carcinoma using anti-DRAM1 antibody [M3-P4B4]. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0)](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/233953e0-dc19-4381-a2fa-7e10493d514f.jpg)
![Survival curve comparing patients outcome categorised by expression of DRAM1 using Anti-DRAM1 [M3-P4B4]. Positive expression of DRAM1 was associated with decrease in patients survival (p=0.039).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/89785f06-3e7d-4027-91b8-1fb8bf523f59.png)
![Image for Anti-DRAM1 [M3-P4B4]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/1f0a774e-bf8f-4328-b635-45aca1d303ae.jpg)
![Image for Anti-DRAM1 [M3-P4B4]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/601fd023-6e43-42b2-8a8a-fae52d472ecc.jpg)
![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue normal colon mucosa, primary tumour and lymph node metastasis sections using anti-DRAM-1 [M3-P4B4].](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/45c76ff1-e785-41e4-b8ac-37b59edbb6e5.jpg)
![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections. Positive cytoplasmic staining is observed in hepatocellualr carcinoma using anti-DRAM1 antibody [M3-P4B4]. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0)](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/233953e0-dc19-4381-a2fa-7e10493d514f.jpg)
![Survival curve comparing patients outcome categorised by expression of DRAM1 using Anti-DRAM1 [M3-P4B4]. Positive expression of DRAM1 was associated with decrease in patients survival (p=0.039).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/89785f06-3e7d-4027-91b8-1fb8bf523f59.png)
![Image thumbnail for Anti-DRAM1 [M3-P4B4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/1f0a774e-bf8f-4328-b635-45aca1d303ae.jpg)
![Image thumbnail for Anti-DRAM1 [M3-P4B4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/601fd023-6e43-42b2-8a8a-fae52d472ecc.jpg)
![Image thumbnail for Anti-DRAM1 [M3-P4B4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/45c76ff1-e785-41e4-b8ac-37b59edbb6e5.jpg)
![Image thumbnail for Anti-DRAM1 [M3-P4B4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/233953e0-dc19-4381-a2fa-7e10493d514f.jpg)
![Image thumbnail for Anti-DRAM1 [M3-P4B4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/89785f06-3e7d-4027-91b8-1fb8bf523f59.png)
| Catalogue Number | 151871 |
| Applications | ELISA IHC IF WB |
| Antigen/Gene or Protein Targets | Damage Regulated Autophagy Modulator 1 (DRAM1) |
| Reactivity | Human |
| Relevance | DRAM1 is significantly upregulated in colorectal cancer and significantly associated with prognosis. |
| Host | Mouse |
| Immunogen | Peptide Sequence - EKDYVYHVVS (amino acids 192 - 201) |
| Positive Control | ELISA- Peptide immunogenWestern Blot- Hela cell extract (cytoplasmic fraction)IF- Hela CellsIHC- Human hepatocellular carcinoma |
| Subclass | IgG1 |
| Myeloma Used | P3X63Ag8.653 |
| Recommended Growing Conditions | Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine + 3ml of Penicillin -Streptomycin. |
| Strain | Balb/c |
| Research Area | Apoptosis and Programmed Cell Death, Cancer, Cardiovascular, Cell Cycle, Cell Signaling & Signal Transduction, Metabolism |
References: 2 entries
Brown et al. 2014. PLoS One. 9(3):e90776. PMID: 24608339.
The expression and prognostic significance of retinoic acid metabolising enzymes in colorectal cancer.
Europe PMC ID: 24608339
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References: 2 entries
Brown et al. 2014. PLoS One. 9(3):e90776. PMID: 24608339.
The expression and prognostic significance of retinoic acid metabolising enzymes in colorectal cancer.
Add a reference
